Detailed information of TA system TA228380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2379824..2380341
• Replicon: chromosome
• Accession: NZ_CP089586
• Organism: Staphylococcus aureus strain E1185_IV_ST12

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: LUU82_RS11760
• Protein ID: WP_000113262.1
• Coordinates: 2380075..2380341 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: LUU82_RS11755
• Protein ID: WP_000584499.1
• Coordinates: 2379824..2380075 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUU82_RS11725 (2375522)	2375522..2376241	+	720	WP_000479576.1	amino acid ABC transporter permease	-
LUU82_RS11730 (2376238)	2376238..2376969	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
LUU82_RS11735 (2377066)	2377066..2378316	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
LUU82_RS11740 (2378571)	2378571..2378933	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
LUU82_RS11745 (2378952)	2378952..2379551	+	600	WP_000162810.1	DsbA family protein	-
LUU82_RS11750 (2379579)	2379579..2379659	+	81	WP_223197974.1	hypothetical protein	-
LUU82_RS11755 (2379824)	2379824..2380075	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LUU82_RS11760 (2380075)	2380075..2380341	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
LUU82_RS11765 (2380376)	2380376..2380447	+	72	WP_223200558.1	hypothetical protein	-
LUU82_RS11770 (2380635)	2380635..2382185	+	1551	WP_000727764.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
LUU82_RS11775 (2382371)	2382371..2382838	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
LUU82_RS11780 (2382925)	2382925..2383107	+	183	WP_000079142.1	hypothetical protein	-
LUU82_RS11785 (2383324)	2383324..2384148	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
LUU82_RS11790 (2384382)	2384382..2384918	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T228380 WP_000113262.1 NZ_CP089586:2380075-2380341 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T228380 NZ_CP089586:2380075-2380341 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT228380 WP_000584499.1 NZ_CP089586:2379824-2380075 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT228380 NZ_CP089586:2379824-2380075 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• AlphaFold DB: T1YB60