Detailed information of TA system TA228377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2323128..2323651
• Replicon: chromosome
• Accession: NZ_CP089586
• Organism: Staphylococcus aureus strain E1185_IV_ST12

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YCD0
• Locus tag: LUU82_RS11485
• Protein ID: WP_000074043.1
• Coordinates: 2323385..2323651 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YBB2
• Locus tag: LUU82_RS11480
• Protein ID: WP_000587618.1
• Coordinates: 2323128..2323385 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUU82_RS11460 (2318210)	2318210..2319403	+	1194	WP_000675406.1	MFS transporter	-
LUU82_RS11465 (2319627)	2319627..2320376	-	750	WP_000072463.1	CPBP family lipoprotein N-acylation protein LnsB	-
LUU82_RS11470 (2320571)	2320571..2322148	+	1578	WP_000143494.1	FMN-binding glutamate synthase family protein	-
LUU82_RS11475 (2322316)	2322316..2322771	-	456	WP_000732091.1	DUF1307 domain-containing protein	-
LUU82_RS11480 (2323128)	2323128..2323385	+	258	WP_000587618.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LUU82_RS11485 (2323385)	2323385..2323651	+	267	WP_000074043.1	Txe/YoeB family addiction module toxin	Toxin
LUU82_RS11490 (2324008)	2324008..2325084	+	1077	WP_001076673.1	M42 family metallopeptidase	-
LUU82_RS11495 (2325332)	2325332..2325994	+	663	WP_000923527.1	ATP-binding cassette domain-containing protein	-
LUU82_RS11500 (2325987)	2325987..2326763	+	777	WP_000072147.1	iron export ABC transporter permease subunit FetB	-
LUU82_RS11505 (2327159)	2327159..2328346	+	1188	WP_232652288.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10657.45 Da
• Isoelectric Point: 10.3584

>T228377 WP_000074043.1 NZ_CP089586:2323385-2323651 [Staphylococcus aureus]
MSNYTVKIKNSAKLDLKKIKHPYLKKTFIEIVETLKNNPYKITQSFEKLEPKYLERYSRRINLQHRVVYTIDDRNKVVLI
LSAWSHYD

Nucleotide

• Length: 267 bp

>T228377 NZ_CP123897:c1003108-1002800 [Methylomonas sp. UP202]
GTGAAAACCGTTTTAACGACCGAGCTTTTCGACCACTGGTTTACCGGCTTGAAAGACATTCAGGCCAAACGTCGTATCCA
AATGCGTATCGACCGTGCCGAGGATGGTCATTTCGGCGATTGCAAACCGGTTGGCGAAGGCGTATCCGAAATGCGCGTCA
ACACCGGTCCGGGTTATCGGATTTATTACACGCAACGTGGAGACGGCTACGAAATCGTTGTACTACTGGCTGGCGGCGAT
AAGTCCTCGCAGGCTCAAGACATCAAGACCGCTATTCAGTTGGCCAGACAAATTAAGGAGCAATCATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9762.78 Da
• Isoelectric Point: 4.1958

>AT228377 WP_000587618.1 NZ_CP089586:2323128-2323385 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETMYLVSTGTMDKVREREKDNSGTTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT228377 NZ_CP123897:c1002803-1002489 [Methylomonas sp. UP202]
ATGACAATCAAATTAAAACGCTGGGATTCGGCCGAGCATTTACAAACCGACGAAGACATCGAATTGTATTTGCAAGCTTG
TTTCGAAGAAGCCGGCGACGACGCCGCCTTTATCGCCAAAGCCTTGGGCAATGTCGCCCGCGCCCGCAGCATGACGCAGT
TGGCCAAGGATACCGGTATGGGGCGGGAAAGTTTATATAAAGCGTTGTCCGGCGAGGGTAATCCAAGTTTCGGCACTATT
TTGAAGGTGACAAATGCATTGGGACTGAAATTTAACGTCCATCTTTCGGATGATTCTGATCGGAAAATGGCTTGA

Structures

Toxin

• AlphaFold DB: T1YCD0

Antitoxin

• AlphaFold DB: T1YBB2