Detailed information of TA system TA228353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3891013..3891667
• Replicon: chromosome
• Accession: NZ_CP089519
• Organism: Pseudomonas extremorientalis strain 1502IPR-01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LUU92_RS17360
• Protein ID: WP_093513166.1
• Coordinates: 3891013..3891363 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1H0L908
• Locus tag: LUU92_RS17365
• Protein ID: WP_071491908.1
• Coordinates: 3891353..3891667 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUU92_RS17350 (LUU92_17320)	3887769..3889301	+	1533	WP_010209575.1	NADH-quinone oxidoreductase subunit M	-
LUU92_RS17355 (LUU92_17325)	3889309..3890772	+	1464	WP_058425023.1	NADH-quinone oxidoreductase subunit NuoN	-
LUU92_RS17360 (LUU92_17330)	3891013..3891363	+	351	WP_093513166.1	toxin	Toxin
LUU92_RS17365 (LUU92_17335)	3891353..3891667	+	315	WP_071491908.1	transcriptional regulator	Antitoxin
LUU92_RS17370 (LUU92_17340)	3892022..3892774	-	753	WP_071491909.1	outer membrane beta-barrel protein	-
LUU92_RS17375 (LUU92_17345)	3892901..3893734	-	834	WP_071491910.1	arylamine N-acetyltransferase	-
LUU92_RS17380 (LUU92_17350)	3893855..3894091	+	237	WP_058425027.1	hypothetical protein	-
LUU92_RS17385 (LUU92_17355)	3894306..3895289	+	984	WP_257102393.1	DUF1852 domain-containing protein	-
LUU92_RS17390 (LUU92_17360)	3895317..3896351	+	1035	WP_071491912.1	methionine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13830.74 Da
• Isoelectric Point: 9.3833

>T228353 WP_093513166.1 NZ_CP089519:3891013-3891363 [Pseudomonas extremorientalis]
MDALFIELPPFERHRKDYLSDELFQGFQQELMKNPEAGDVIEGTGGLRKVRFIDERRNKGKRGGLRVIYYWWSGGTQFWL
FTLYGKHEQDDLTPQHKKALKHMLDREIKARTHHET

Nucleotide

• Length: 351 bp

>T228353 NZ_CP123849:348974-349393 [Agrobacterium fabacearum]
ATGATCTTGCTTGACACCAACGTCATATCCGAACCTTGGAAACCGGTTCCGGACGAGGCGGTGATTGCCTGGTTGGACGC
CCAAGCTGTCGAAACGCTGTTCATCTCCGCAATAACGATTGCGGAACTTCGCTTCGGAATTGCTGCTATGCCTTCAGGCA
GACGGCAAACTATCCTCCGCGACCGTCTCGAGGGCGAGGTGCTGCCACACTTTTCCGGCCGCATCCTGTCCTTCGATCTG
ACTACTTCTCAGTTCTACTCTGAACTGATGGCACGCGCTCGAGCGTCTGGGAAAGCAATCGGCACGGCCGATGGCTACAT
TGCAGCCACAGCGGCAGCAAATGGCCTCACGATCTCAACTCGCGATACAAGCCCTTTCGAGGCTGCCGGGGTGAAAGTGA
TCAATCCCTGGTCCAGATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11771.53 Da
• Isoelectric Point: 10.4449

>AT228353 WP_071491908.1 NZ_CP089519:3891353-3891667 [Pseudomonas extremorientalis]
MKREIFSELVEGFDALANERQGKVTLRTHKVHLNQLEPLTAEEVVAVRQQLNLSRSVFAMYLRTNTRTLENWEQGRAKPN
AQAATLIRLVARFPETVAQLAALG

Nucleotide

• Length: 315 bp

>AT228353 NZ_CP123849:348723-348977 [Agrobacterium fabacearum]
ATGCCGGCAGTCACCATTCGTAACCTTTCTGAAGAAACACATCGCGCTCTGAGGGTGCGAGCGGCTCATCATGGCCGGAG
CACGGAGGCCGAGATTCGTGACATTATTGAAGCGGCTGTTCGTCCTCCGGAACGTATTCAGCTCGGGTCGTTGCTAGCGG
CCATTGGTCGCGACGCCGAGCTTTCCAACAGCGACGTTGAAGCTCTTCAGAAAAGTCGCGACAAGACACCTGCTAAGCCG
ATGACCTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1H0L908