Detailed information of TA system TA228339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3833431..3834028
• Replicon: chromosome
• Accession: NZ_CP089509
• Organism: Klebsiella pneumoniae strain GD21SC417

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: LT986_RS18960
• Protein ID: WP_004893639.1
• Coordinates: 3833711..3834028 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: LT986_RS18955
• Protein ID: WP_004142561.1
• Coordinates: 3833431..3833718 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT986_RS18925 (3829638)	3829638..3829886	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
LT986_RS18930 (3829904)	3829904..3830245	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
LT986_RS18935 (3830276)	3830276..3831391	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
LT986_RS18940 (3831571)	3831571..3832152	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
LT986_RS18945 (3832152)	3832152..3832520	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
LT986_RS18950 (3832640)	3832640..3833293	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
LT986_RS18955 (3833431)	3833431..3833718	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
LT986_RS18960 (3833711)	3833711..3834028	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LT986_RS18965 (3834213)	3834213..3835256	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
LT986_RS18970 (3835926)	3835926..3836792	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
LT986_RS18975 (3836901)	3836901..3838328	+	1428	WP_105910454.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T228339 WP_004893639.1 NZ_CP089509:c3834028-3833711 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T228339 NZ_CP123783:720405-720704 [Minwuia sp. IMCC3077]
ATGCCGGATAGCGATCCGGGCTACCGGCTTTCTCCGCTCGCGAGAACCGATCTGGAAGACATCTGGCGCTACACCTTCGA
GAACTGGTCGCTTGAACAGGCGGATCGCTACCATGGCACGATCATGACCGCGATCCTGGCGTTGGCTGATGGCTCAGTAT
CGGGTAGCCGCGCCGACGTCCGTGAGGGCTATTTCAAGCTAAGAGCCGGTTCCCATGTCCTGTTCTACCGAAAGGCCGAC
ACCACTCTGGAGATCATGCGCATCCTGCATCAGCGCATGGACGTGGATCGACACCTCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT228339 WP_004142561.1 NZ_CP089509:c3833718-3833431 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT228339 NZ_CP123783:720170-720415 [Minwuia sp. IMCC3077]
ATGGCACGCAATACCTCGGTCTCGCTTGGTGATCATTTCACCGGCTTCATTGACAGTCAGGTTCGGGACGGTCGCTACGG
CTCCGCCAGCGATGTGGTCAGGGCTGGTCTGCGGCTGCTGGAGGAACATGAAACCAGGGTCCGAGCCTTGCAGAACGCCC
TGATCGCCGCAGAGGAATCCGGTCCCCCAGAAGCTTTCGACAGCGATGCCTTCCTGGAGGAAATGCGGAAGAAGCATGCC
GGATAG

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1