Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 40265..40915 Replicon chromosome
Accession NZ_CP089448
Organism Klebsiella michiganensis strain 8-1

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag LT930_RS00195 Protein ID WP_014227314.1
Coordinates 40265..40606 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag LT930_RS00200 Protein ID WP_014227313.1
Coordinates 40616..40915 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT930_RS00180 (LT930_00180) 36528..37847 + 1320 WP_014227316.1 MFS transporter -
LT930_RS00185 (LT930_00185) 37993..39384 + 1392 WP_064375119.1 hexose-6-phosphate:phosphate antiporter -
LT930_RS00190 (LT930_00190) 39562..40014 + 453 WP_009653860.1 DUF1198 domain-containing protein -
LT930_RS00195 (LT930_00195) 40265..40606 + 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LT930_RS00200 (LT930_00200) 40616..40915 + 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
LT930_RS00205 (LT930_00205) 41034..42215 + 1182 WP_201161991.1 purine ribonucleoside efflux pump NepI -
LT930_RS00210 (LT930_00210) 42248..43057 - 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
LT930_RS00215 (LT930_00215) 43067..44170 - 1104 WP_025107574.1 2-aminoethylphosphonate--pyruvate transaminase -
LT930_RS00220 (LT930_00220) 44311..45030 + 720 WP_009653851.1 phosphonate utilization transcriptional regulator PhnR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T228309 WP_014227314.1 NZ_CP089448:40265-40606 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T228309 NZ_CP123660:4608328-4608639 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCCCTCACTGAGTTGCT
GAATGTTCTTGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAAACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT228309 WP_014227313.1 NZ_CP089448:40616-40915 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT228309 NZ_CP123660:4608636-4609082 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAGAACTCATCGCCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTACTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACATCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References