Detailed information of TA system TA228308

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 124054..124307
• Replicon: plasmid unnamed2
• Accession: NZ_CP089447
• Organism: Escherichia coli strain 5-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LT922_RS26665
• Protein ID: WP_001312851.1
• Coordinates: 124158..124307 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	124054..124113 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT922_RS26625 (119714)	119714..119779	-	66	Protein_162	helix-turn-helix domain-containing protein	-
LT922_RS26630 (119832)	119832..120536	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LT922_RS26635 (120561)	120561..120761	+	201	WP_072354025.1	hypothetical protein	-
LT922_RS26640 (120781)	120781..121527	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LT922_RS26645 (121582)	121582..122142	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LT922_RS26650 (122274)	122274..122474	+	201	WP_015059022.1	hypothetical protein	-
LT922_RS26655 (122860)	122860..123459	+	600	WP_032083981.1	PIN domain-containing protein	-
LT922_RS26660 (123521)	123521..123853	+	333	WP_152916585.1	hypothetical protein	-
- (124054)	124054..124113	-	60	NuclAT_1	-	Antitoxin
- (124054)	124054..124113	-	60	NuclAT_1	-	Antitoxin
- (124054)	124054..124113	-	60	NuclAT_1	-	Antitoxin
- (124054)	124054..124113	-	60	NuclAT_1	-	Antitoxin
LT922_RS26665 (124158)	124158..124307	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LT922_RS26670 (124591)	124591..124839	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	fosA3 / blaTEM-1B / rmtB / blaKPC-2 / blaSHV-12 / catA2	-	1..125150	125150

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T228308 WP_001312851.1 NZ_CP089447:124158-124307 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T228308 NZ_CP123660:c4607095-4606784 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT228308 NZ_CP089447:c124113-124054 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin