Detailed information of TA system TA228304

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 32300..32569
• Replicon: plasmid unnamed2
• Accession: NZ_CP089447
• Organism: Escherichia coli strain 5-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LT922_RS26035
• Protein ID: WP_001372321.1
• Coordinates: 32444..32569 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	32300..32365 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT922_RS26000	28010..28537	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LT922_RS26005	28595..28828	+	234	WP_000006003.1	DUF905 family protein	-
LT922_RS26010	28889..30912	+	2024	Protein_39	ParB/RepB/Spo0J family partition protein	-
LT922_RS26015	30981..31415	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LT922_RS26020	31412..32174	+	763	Protein_41	plasmid SOS inhibition protein A	-
-	32143..32367	+	225	NuclAT_0	-	-
-	32143..32367	+	225	NuclAT_0	-	-
-	32143..32367	+	225	NuclAT_0	-	-
-	32143..32367	+	225	NuclAT_0	-	-
LT922_RS26025	32152..32331	-	180	WP_001309233.1	hypothetical protein	-
-	32300..32365	-	66	-	-	Antitoxin
LT922_RS26030	32353..32502	+	150	Protein_43	plasmid maintenance protein Mok	-
LT922_RS26035	32444..32569	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LT922_RS26040	32888..33184	-	297	Protein_45	hypothetical protein	-
LT922_RS26045	33535..34239	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LT922_RS26050	34302..34697	+	396	Protein_47	ATP-dependent endonuclease	-
LT922_RS26055	34682..35704	+	1023	WP_000361404.1	helicase UvrD	-
LT922_RS26060	35960..36657	+	698	WP_223174630.1	IS1 family transposase	-
LT922_RS26065	36686..36958	+	273	Protein_50	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	fosA3 / blaTEM-1B / rmtB / blaKPC-2 / blaSHV-12 / catA2	-	1..125150	125150

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T228304 WP_001372321.1 NZ_CP089447:32444-32569 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T228304 NZ_CP123660:3653616-3653843 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGACCACCTATTACTCACGCATTCCCAGCCTGCATCTCAAGGGTGACTGGCTGACAGAAGCGGGATTTGATACCGGCGC
GAGCGTGACAGTGAAGATCTCCGAGGGGTGTTTGATCCTGATTGCAGAGACGGACGAAGTGAGGGATTTACGGAAGGAGC
TTTATCAGGTGAAAAAGTCGATGAAGCATATTAAGGCGGGGGTAAATAATGTGGTGAACGGGAATTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT228304 NZ_CP089447:c32365-32300 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin