Detailed information of TA system TA228303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5082570..5083172
• Replicon: chromosome
• Accession: NZ_CP089445
• Organism: Escherichia coli strain 5-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: LT922_RS24860
• Protein ID: WP_000897302.1
• Coordinates: 5082570..5082881 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LT922_RS24865
• Protein ID: WP_000356397.1
• Coordinates: 5082882..5083172 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT922_RS24835 (5078484)	5078484..5079083	+	600	WP_001296610.1	glucose-1-phosphatase	-
LT922_RS24840 (5079077)	5079077..5079949	+	873	WP_000920762.1	virulence factor BrkB family protein	-
LT922_RS24845 (5079946)	5079946..5080383	+	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
LT922_RS24850 (5080428)	5080428..5081369	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LT922_RS24855 (5081433)	5081433..5082341	-	909	WP_001385591.1	alpha/beta hydrolase	-
LT922_RS24860 (5082570)	5082570..5082881	+	312	WP_000897302.1	hypothetical protein	Toxin
LT922_RS24865 (5082882)	5082882..5083172	+	291	WP_000356397.1	NadS family protein	Antitoxin
LT922_RS24870 (5083531)	5083531..5083809	+	279	WP_001296612.1	hypothetical protein	-
LT922_RS24875 (5084206)	5084206..5084424	+	219	WP_001251293.1	CopG family transcriptional regulator	-
LT922_RS24880 (5084609)	5084609..5085349	-	741	WP_000608806.1	hypothetical protein	-
LT922_RS24885 (5085374)	5085374..5086222	-	849	WP_001038650.1	hypothetical protein	-
LT922_RS24890 (5086512)	5086512..5086754	+	243	WP_001068514.1	CopG family transcriptional regulator	-
LT922_RS24895 (5086936)	5086936..5087865	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T228303 WP_000897302.1 NZ_CP089445:5082570-5082881 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T228303 NZ_CP123660:3651284-3651529 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCGG
CGACTGCCAGGAAGAAGCGGGATTTGAGACCGGGCGCAGCGTGACGGTGAAGATTTCGGACGGGTGTATTGTGCTAATGG
CGGACGGTAACGAGGTGCAGAAGCTGTGCGAACAGCTTTATCAGGCTGAACAGGTAGTTAAGGGGATGTGGGACGTGATT
GTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT228303 WP_000356397.1 NZ_CP089445:5082882-5083172 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT228303 NZ_CP123660:c3651493-3651127 [Salmonella enterica subsp. enterica serovar Kentucky]
TTCAGCCTGATAAAGCTGTTCGCACAGCTTCTGCACCTCGTTACCGTCCGCCATTAGCACAATACACCCGTCCGAAATCT
TCACCGTCACGCTGCGCCCGGTCTCAAATCCCGCTTCTTCCTGGCAGTCGCCGCTGGGGATTTGTGAGTAACAGGCGGTC
ATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATC
CGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACTGCGCCCCCCTCTCCCCG
GCCAGCACCGGGCGGTGATGAGCAACCGGCTGCCGGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: S1P416