Detailed information of TA system TA228301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4273357..4274156
• Replicon: chromosome
• Accession: NZ_CP089445
• Organism: Escherichia coli strain 5-1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: LT922_RS20910
• Protein ID: WP_000347251.1
• Coordinates: 4273692..4274156 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: LT922_RS20905
• Protein ID: WP_001296435.1
• Coordinates: 4273357..4273692 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT922_RS20890 (4269142)	4269142..4269912	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
LT922_RS20895 (4269928)	4269928..4271262	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
LT922_RS20900 (4271637)	4271637..4273208	+	1572	WP_001273763.1	galactarate dehydratase	-
LT922_RS20905 (4273357)	4273357..4273692	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
LT922_RS20910 (4273692)	4273692..4274156	+	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
LT922_RS20915 (4274211)	4274211..4275020	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
LT922_RS20920 (4275269)	4275269..4276549	+	1281	WP_000681950.1	tagatose-bisphosphate aldolase subunit KbaZ	-
LT922_RS20925 (4276572)	4276572..4277045	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
LT922_RS20930 (4277056)	4277056..4277835	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
LT922_RS20935 (4277825)	4277825..4278703	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
LT922_RS20940 (4278721)	4278721..4279155	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T228301 WP_000347251.1 NZ_CP089445:4273692-4274156 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T228301 NZ_CP123660:2438163-2438447 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT228301 WP_001296435.1 NZ_CP089445:4273357-4273692 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT228301 NZ_CP123660:2437925-2438173 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20

Antitoxin

• AlphaFold DB: S1PPV5