Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4273357..4274156 Replicon chromosome
Accession NZ_CP089445
Organism Escherichia coli strain 5-1

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag LT922_RS20910 Protein ID WP_000347251.1
Coordinates 4273692..4274156 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag LT922_RS20905 Protein ID WP_001296435.1
Coordinates 4273357..4273692 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT922_RS20890 (4269142) 4269142..4269912 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
LT922_RS20895 (4269928) 4269928..4271262 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
LT922_RS20900 (4271637) 4271637..4273208 + 1572 WP_001273763.1 galactarate dehydratase -
LT922_RS20905 (4273357) 4273357..4273692 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LT922_RS20910 (4273692) 4273692..4274156 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LT922_RS20915 (4274211) 4274211..4275020 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LT922_RS20920 (4275269) 4275269..4276549 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
LT922_RS20925 (4276572) 4276572..4277045 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LT922_RS20930 (4277056) 4277056..4277835 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LT922_RS20935 (4277825) 4277825..4278703 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LT922_RS20940 (4278721) 4278721..4279155 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T228301 WP_000347251.1 NZ_CP089445:4273692-4274156 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T228301 NZ_CP123660:2438163-2438447 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT228301 WP_001296435.1 NZ_CP089445:4273357-4273692 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT228301 NZ_CP123660:2437925-2438173 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References