Detailed information of TA system TA228296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2943432..2943934
• Replicon: chromosome
• Accession: NZ_CP089445
• Organism: Escherichia coli strain 5-1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: LT922_RS14700
• Protein ID: WP_000767819.1
• Coordinates: 2943432..2943686 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: LT922_RS14705
• Protein ID: WP_001259253.1
• Coordinates: 2943683..2943934 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT922_RS14675 (2939250)	2939250..2939708	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
LT922_RS14680 (2939925)	2939925..2941283	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
LT922_RS14685 (2941273)	2941273..2941335	-	63	WP_010723108.1	membrane protein YoeI	-
LT922_RS14690 (2941550)	2941550..2942479	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
LT922_RS14695 (2942525)	2942525..2943349	-	825	WP_000754737.1	SDR family oxidoreductase	-
LT922_RS14700 (2943432)	2943432..2943686	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
LT922_RS14705 (2943683)	2943683..2943934	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LT922_RS14710 (2944217)	2944217..2944267	+	51	WP_001364200.1	his operon leader peptide	-
LT922_RS14715 (2944413)	2944413..2945312	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
LT922_RS14720 (2945318)	2945318..2946622	+	1305	WP_001296211.1	histidinol dehydrogenase	-
LT922_RS14725 (2946619)	2946619..2947689	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
LT922_RS14730 (2947689)	2947689..2948756	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2939250..2939708	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T228296 WP_000767819.1 NZ_CP089445:c2943686-2943432 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T228296 NZ_CP123642:4820516-4820926 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT228296 WP_001259253.1 NZ_CP089445:c2943934-2943683 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT228296 NZ_CP123642:4820269-4820535 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3