Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1283013..1283692 Replicon chromosome
Accession NZ_CP089445
Organism Escherichia coli strain 5-1

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag LT922_RS06155 Protein ID WP_000057523.1
Coordinates 1283013..1283315 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag LT922_RS06160 Protein ID WP_000806442.1
Coordinates 1283351..1283692 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT922_RS06130 (1278389) 1278389..1280041 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
LT922_RS06135 (1280079) 1280079..1280582 - 504 WP_000667000.1 hypothetical protein -
LT922_RS06140 (1280579) 1280579..1281235 - 657 WP_015674862.1 hypothetical protein -
LT922_RS06145 (1281403) 1281403..1281882 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
LT922_RS06150 (1282086) 1282086..1282880 - 795 WP_000365147.1 TraB/GumN family protein -
LT922_RS06155 (1283013) 1283013..1283315 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LT922_RS06160 (1283351) 1283351..1283692 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
LT922_RS06165 (1283750) 1283750..1286254 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
LT922_RS06170 (1286516) 1286516..1287448 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T228281 WP_000057523.1 NZ_CP089445:1283013-1283315 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T228281 NZ_CP123618:1670049-1670339 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTGAAGCCATTTGATATTGCTGAACATCTAGATAGTGAAGAAATGATTGCCGCATATTTAAG
TGAAGTTTTAGAAACAGGTGATACTAATGAATTTATTTCCGCGCTAGGTGATGTTGCAAGAGCAAGAGGAATGACGGAGT
TAGCAGCAAAAACAGGGTTGGGAAGAGAAAGTCTTTATAAAACATTATCATACGGTAGTAAGCCACGTTTTGATACTATA
ATGAAAATTACGCAAGCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT228281 WP_000806442.1 NZ_CP089445:1283351-1283692 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT228281 NZ_CP123618:1669738-1670052 [Pasteurella multocida]
ATGTTTGAGCTAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAAGATTTGTCGGCTAAGGCGGCTATTCT
TGCTCGTATTAATAGAGCTAAAAATGGTAATTTTGGCGATCACAAATCAGTTGGTGATGGCTTGTATGAAATGAGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTACACACAATACAAAGATGTAACCTATTTGTTAATTTGCGGAGGTGATAAATCT
ACACAGAAAGCAGACATCGCTAAAGCAAAAGTTTTATGGGAAGAGATTAAGCAGAAAGAGGGGGTAAATGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References