Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 5827018..5827668 Replicon chromosome
Accession NZ_CP089395
Organism Klebsiella michiganensis strain Kox58

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag LUW95_RS27630 Protein ID WP_014227314.1
Coordinates 5827327..5827668 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag LUW95_RS27625 Protein ID WP_014227313.1
Coordinates 5827018..5827317 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUW95_RS27605 (LUW95_27605) 5822903..5823622 - 720 WP_232421365.1 phosphonate utilization transcriptional regulator PhnR -
LUW95_RS27610 (LUW95_27610) 5823763..5824866 + 1104 WP_232421366.1 2-aminoethylphosphonate--pyruvate transaminase -
LUW95_RS27615 (LUW95_27615) 5824876..5825685 + 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
LUW95_RS27620 (LUW95_27620) 5825718..5826899 - 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
LUW95_RS27625 (LUW95_27625) 5827018..5827317 - 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
LUW95_RS27630 (LUW95_27630) 5827327..5827668 - 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LUW95_RS27635 (LUW95_27635) 5827919..5828371 - 453 WP_009653860.1 DUF1198 domain-containing protein -
LUW95_RS27640 (LUW95_27640) 5828820..5830211 - 1392 WP_232421367.1 hexose-6-phosphate:phosphate antiporter -
LUW95_RS27645 (LUW95_27645) 5830357..5831676 - 1320 WP_032751824.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5800878..5827668 26790


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T228165 WP_014227314.1 NZ_CP089395:c5827668-5827327 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T228165 NZ_CP123373:c155925-155593 [Providencia rettgeri]
ATGGTATCTCGCTACGTGCCTGATTCTGGCGATTTGATTTGGATTGACTTTGATCCAGTTGAAGGACATGAGCAGGGCGG
TCATCGTCCAGCAGTTGTCCTCAGCCCATTTGCATACAATAATAAAGTAGGACTCTTATTATGCGTACCATGTACAACTA
AGGGCAAAGGCTATCCATTTGAATGCGAACTCTCGAACGAAAGAGACAGCTACGCATTAGCGGATCAAGTTACATGTGTT
GACTGGAGAGCTCGTAATGTTACTAAAAAGGGCAAAGTGGAGGCATCAGAACTGGCAGAGATAAAAGCTAAAGCAAAGGC
GTTAATAGGTTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT228165 WP_014227313.1 NZ_CP089395:c5827317-5827018 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT228165 NZ_CP123373:c156170-155925 [Providencia rettgeri]
ATGGCTCAGGTCACAGTAAAAAAATGGGGCAATAGCCCGTCTGTACGGCTTCCTGTTGCAATCATGCAAAAAGCGGCACT
TAGCGTTGATGATACGGTTGAAATAGCGGTAGAAGAAGGTCGTATCATCATTACGCCAGTTAAAGCGGTAGAATACTCAT
TAGAAAATTTGTTAGCCGGTATCACGCCGGAGAATATTCATGAGAAAGTAGATTTCGGAGCTCGCACAGGTAAGGAATTA
ATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References