Detailed information of TA system TA228110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2643789..2644392
• Replicon: chromosome
• Accession: NZ_CP089335
• Organism: Eggerthella lenta strain APC055-924-7B

Toxin (Protein)

• Gene name: graT
• Uniprot ID: C8WPE4
• Locus tag: LT426_RS11360
• Protein ID: WP_015760380.1
• Coordinates: 2644111..2644392 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: LT426_RS11355
• Protein ID: WP_226844067.1
• Coordinates: 2643789..2644085 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT426_RS11340	2641060..2641512	+	453	WP_226844136.1	sigma-70 family RNA polymerase sigma factor	-
LT426_RS11345	2641502..2642098	+	597	WP_015760383.1	hypothetical protein	-
LT426_RS11350	2642120..2643787	+	1668	WP_015760382.1	VWA domain-containing protein	-
LT426_RS11355	2643789..2644085	-	297	WP_226844067.1	HigA family addiction module antitoxin	Antitoxin
LT426_RS11360	2644111..2644392	-	282	WP_015760380.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LT426_RS11365	2644424..2645740	-	1317	WP_015760379.1	amidohydrolase family protein	-
LT426_RS11370	2645805..2646362	-	558	WP_015760378.1	GNAT family N-acetyltransferase	-
LT426_RS11375	2646359..2646991	-	633	WP_015760377.1	7-cyano-7-deazaguanine synthase	-
LT426_RS11380	2646988..2648112	-	1125	WP_015760376.1	aldo/keto reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10537.99 Da
• Isoelectric Point: 7.2921

>T228110 WP_015760380.1 NZ_CP089335:c2644392-2644111 [Eggerthella lenta]
MIKTFADKDTERLFTDGSCKRISSDVAARTARKLDMIDAALTLEDLRVPPGNRLHALKGDRAGMHAISVNDQWRLCFRFE
GQHAYDVALCDYH

Nucleotide

• Length: 282 bp

>T228110 NZ_CP123282:c4206468-4205977 [Salmonella sp. SA14318]
ATGATCTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11285.01 Da
• Isoelectric Point: 5.7378

>AT228110 WP_226844067.1 NZ_CP089335:c2644085-2643789 [Eggerthella lenta]
MILMARKPTHPGEVLREEFMPDYGLTVASLAKRLGVSRQSVNEVVRERRAVSTEMAMRLSRLFGTSAEYWLNLQRNVDLW
ESLDLHREELDAIEALIA

Nucleotide

• Length: 297 bp

>AT228110 NZ_CP123282:c4206758-4206465 [Salmonella sp. SA14318]
ATGCCAGCCGCAAACAGTATGGCGATGAAGCGTGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCACAGGCTTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: C8WPE4