Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 6368..7095 Replicon plasmid pKP062-1
Accession NZ_CP089326
Organism Klebsiella pneumoniae strain KP062

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag LUX41_RS25815 Protein ID WP_011251285.1
Coordinates 6368..6679 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LUX41_RS25820 Protein ID WP_011251286.1
Coordinates 6676..7095 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUX41_RS25785 (LUX41_25750) 1742..1948 + 207 Protein_1 integrase core domain-containing protein -
LUX41_RS25790 (LUX41_25755) 2967..3814 + 848 Protein_2 IS5 family transposase -
LUX41_RS25795 (LUX41_25760) 3832..4881 - 1050 Protein_3 IS66 family transposase -
LUX41_RS25800 (LUX41_25765) 4930..5277 - 348 WP_004114612.1 IS66 family insertion sequence element accessory protein TnpB -
LUX41_RS25805 (LUX41_25770) 5274..5612 - 339 WP_047683432.1 transposase -
LUX41_RS25810 (LUX41_25775) 5726..6163 + 438 Protein_6 DDE-type integrase/transposase/recombinase -
LUX41_RS25815 (LUX41_25780) 6368..6679 + 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
LUX41_RS25820 (LUX41_25785) 6676..7095 + 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
LUX41_RS25825 (LUX41_25790) 7242..8208 + 967 Protein_9 IS5 family transposase -
LUX41_RS25830 (LUX41_25795) 8545..9513 + 969 WP_077256550.1 IS5-like element IS903B family transposase -
LUX41_RS25835 (LUX41_25800) 9559..10104 - 546 WP_277738958.1 hypothetical protein -
LUX41_RS25840 (LUX41_25805) 10104..10568 - 465 WP_117055985.1 conjugative transfer protein MobI(A/C) -
LUX41_RS25845 (LUX41_25810) 11162..11545 + 384 WP_224238761.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..172904 172904
- inside IScluster/Tn - - 1..24125 24124


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T228104 WP_011251285.1 NZ_CP089326:6368-6679 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T228104 NZ_CP123282:2000267-2000370 [Salmonella sp. SA14318]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT228104 WP_011251286.1 NZ_CP089326:6676-7095 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT228104 NZ_CP123282:c2000372-2000229 [Salmonella sp. SA14318]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1

Antitoxin

Source ID Structure

References