Detailed information of TA system TA022804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 457447..458014
• Replicon: chromosome
• Accession: NC_010577
• Organism: Xylella fastidiosa M23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q87EE4
• Locus tag: XFASM23_RS01930
• Protein ID: WP_004090695.1
• Coordinates: 457447..457728 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: XFASM23_RS01935
• Protein ID: WP_004085172.1
• Coordinates: 457739..458014 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XFASM23_RS01905	453870..454589	-	720	Protein_350	phage late control D family protein	-
XFASM23_RS01910	454489..455460	-	972	WP_011097609.1	hypothetical protein	-
XFASM23_RS01915	455468..456025	-	558	WP_011097610.1	phage tail protein I	-
XFASM23_RS01920	456018..456911	-	894	WP_011097611.1	baseplate J/gp47 family protein	-
XFASM23_RS01925	456911..457249	-	339	WP_004090694.1	GPW/gp25 family protein	-
XFASM23_RS01930	457447..457728	+	282	WP_004090695.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XFASM23_RS01935	457739..458014	+	276	WP_004085172.1	HigA family addiction module antidote protein	Antitoxin
XFASM23_RS01940	458102..458404	+	303	WP_004090696.1	type II toxin-antitoxin system MqsR family toxin	-
XFASM23_RS01945	458407..458808	+	402	WP_004090697.1	type II toxin-antitoxin system MqsA family antitoxin	-
XFASM23_RS01950	458877..459464	-	588	WP_011097612.1	phage baseplate assembly protein V	-
XFASM23_RS01955	459461..460003	-	543	WP_011097613.1	hypothetical protein	-
XFASM23_RS01960	459979..460500	-	522	WP_011097614.1	hypothetical protein	-
XFASM23_RS01965	460497..460820	-	324	WP_011097935.1	hypothetical protein	-
XFASM23_RS01970	460820..461080	-	261	WP_011097616.1	hypothetical protein	-
XFASM23_RS01975	461098..462972	-	1875	WP_011097617.1	phage major capsid protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	446054..468878	22824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10639.21 Da
• Isoelectric Point: 10.0980

>T22804 WP_004090695.1 NC_010577:457447-457728 [Xylella fastidiosa M23]
MIKSFRHKGIQQFFLKGSTAGIQTKHAAKLRIQLTALESAKRPEDMNAPGWKLHPLKGADLKGHWSIWVNGNYRLTFAFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T22804 NC_010577:457447-457728 [Xylella fastidiosa M23]
ATGATCAAAAGTTTCCGCCACAAAGGCATTCAACAATTCTTCCTCAAGGGATCAACCGCTGGTATCCAAACCAAACACGC
CGCCAAACTGCGTATCCAGCTCACCGCGTTGGAGAGTGCCAAGCGCCCCGAAGATATGAATGCTCCAGGCTGGAAACTGC
ACCCACTAAAAGGCGCTGACTTAAAAGGTCATTGGTCCATCTGGGTGAATGGCAACTATCGCCTCACGTTTGCTTTTGAA
GGCGAAGACGCAATCTTGGTCGACTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10072.68 Da
• Isoelectric Point: 10.2184

>AT22804 WP_004085172.1 NC_010577:457739-458014 [Xylella fastidiosa M23]
MKRMHNPAHPGEVLREYLGELTVTNAAAKLGVSRVALSRILNGTNGISADMALRLEDALGTSAEMWAAMQFKYDLWVASQ
QPRPKITRLHV

Nucleotide

• Length: 276 bp

>AT22804 NC_010577:457739-458014 [Xylella fastidiosa M23]
ATGAAACGTATGCACAATCCCGCACATCCAGGTGAAGTGTTGCGTGAGTATCTTGGTGAGCTCACTGTGACCAATGCGGC
TGCAAAGCTAGGCGTCAGCCGTGTGGCTTTGTCGCGTATTCTCAATGGTACCAATGGCATCTCTGCCGATATGGCCCTTC
GCCTGGAAGATGCGCTTGGCACCAGTGCAGAGATGTGGGCAGCCATGCAGTTCAAGTATGACCTGTGGGTTGCTAGTCAG
CAGCCACGCCCGAAGATCACGCGCCTTCATGTTTAG

Structures

Toxin

• AlphaFold DB: Q87EE4