Detailed information of TA system TA228000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-DinJ
• Location: 1379525..1380089
• Replicon: chromosome
• Accession: NZ_CP089307
• Organism: Lacticaseibacillus paracasei strain VHProbi F22

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A6G9EML4
• Locus tag: MTX54_RS07010
• Protein ID: WP_014951859.1
• Coordinates: 1379787..1380089 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: K6SLT0
• Locus tag: MTX54_RS07005
• Protein ID: WP_003570051.1
• Coordinates: 1379525..1379794 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTX54_RS06975 (LT340_06975)	1374529..1375416	-	888	WP_012491506.1	ABC transporter ATP-binding protein	-
MTX54_RS06980 (LT340_06980)	1375614..1376840	-	1227	WP_012491507.1	hypothetical protein	-
MTX54_RS06985 (LT340_06985)	1377038..1377442	+	405	WP_012491508.1	MerR family transcriptional regulator	-
MTX54_RS06990 (LT340_06990)	1377435..1377761	+	327	WP_012491509.1	YrdB family protein	-
MTX54_RS06995 (LT340_06995)	1377824..1378453	+	630	WP_012491510.1	DUF2399 domain-containing protein	-
MTX54_RS07000 (LT340_07000)	1378498..1379151	+	654	WP_012491511.1	N-acetyltransferase	-
MTX54_RS07005 (LT340_07005)	1379525..1379794	+	270	WP_003570051.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MTX54_RS07010 (LT340_07010)	1379787..1380089	+	303	WP_014951859.1	type II toxin-antitoxin system YafQ family toxin	Toxin
MTX54_RS07015 (LT340_07015)	1380428..1380571	+	144	WP_003574774.1	hypothetical protein	-
MTX54_RS07020 (LT340_07020)	1380689..1381381	+	693	WP_012491513.1	hypothetical protein	-
MTX54_RS07025 (LT340_07025)	1381617..1383794	+	2178	WP_012491514.1	Tex family protein	-
MTX54_RS07030 (LT340_07030)	1383859..1384788	-	930	WP_003599410.1	IS30-like element ISLpl1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1383859..1384788	929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-98)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (10-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11441.27 Da
• Isoelectric Point: 10.2818

>T228000 WP_014951859.1 NZ_CP089307:1379787-1380089 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIK
QQDLILTLVRTGSHHNLLGK

Nucleotide

• Length: 303 bp

>T228000 NZ_CP123251:c4443075-4442791 [Providencia rettgeri]
ATGAATACTAAATTAAAAGAATTTGATATCGCTGAGTATTTAAACAGTGAAGAAGAAATACAAATGTATCTAAACGAAAT
TCTAGAAGAAGGTGACCCTAGTCTAGTTCTCGCTGCACTTGGTGATATTGCGAAGGCAAGAAATATGAGCCAATTAGCTA
AAGAAGTTGGAATGAGTCGTGAAGGTTTATATAAGGCGCTATCAGGACAGGGAAATCCAACATTTATGACGATTATAAAA
ATCGTTAATTCTCTCGGATTAAAGCTACAGTTTAAGAGTATCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9982.68 Da
• Isoelectric Point: 7.2026

>AT228000 WP_003570051.1 NZ_CP089307:1379525-1379794 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Nucleotide

• Length: 270 bp

>AT228000 NZ_CP123251:c4443374-4443072 [Providencia rettgeri]
ATGATCACGGTTCTAACAACGGAATGTTTTGATAGTTGGATTAAAAACCTTAGAGATATTCGAGCTAAAACTAAAATTCT
AATGAGAATTAGGCGGTTAAAAAATGGAAATTATGGTGATGTTCAACCCATTGGTGATGGCTTTTCGGAACTGAGGGTAC
ATGAAGGACAAGGGTATCGTGTTTATCTAAAGCAGAAAAATAATGTTATTGTAATTTTACTTTGTGGTGGGACAAAGGCA
ACGCAGCAGAAGGATATCCATAAAGCTAAACTTCTTTTTGGTGAAGTTGAGGGGGAACTATGA

Structures

Toxin

• AlphaFold DB: A0A6G9EML4

Antitoxin

• AlphaFold DB: A0A0E2BRK6