Detailed information of TA system TA227967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4483679..4484373
• Replicon: chromosome
• Accession: NZ_CP089272
• Organism: Escherichia coli O157:H7 strain M1300706001A

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: LSI73_RS22905
• Protein ID: WP_001263495.1
• Coordinates: 4483679..4484077 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: LSI73_RS22910
• Protein ID: WP_000554757.1
• Coordinates: 4484080..4484373 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4479266)	4479266..4479346	-	81	NuclAT_11	-	-
- (4479266)	4479266..4479346	-	81	NuclAT_11	-	-
- (4479266)	4479266..4479346	-	81	NuclAT_11	-	-
- (4479266)	4479266..4479346	-	81	NuclAT_11	-	-
LSI73_RS22880 (4479942)	4479942..4480400	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LSI73_RS22885 (4480661)	4480661..4482118	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
LSI73_RS22890 (4482175)	4482175..4482696	-	522	Protein_4487	peptide chain release factor H	-
LSI73_RS22895 (4482695)	4482695..4482898	-	204	Protein_4488	RtcB family protein	-
LSI73_RS22900 (4483217)	4483217..4483669	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
LSI73_RS22905 (4483679)	4483679..4484077	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LSI73_RS22910 (4484080)	4484080..4484373	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LSI73_RS22915 (4484425)	4484425..4485480	-	1056	WP_001226186.1	DNA polymerase IV	-
LSI73_RS22920 (4485551)	4485551..4486336	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
LSI73_RS22925 (4486308)	4486308..4488020	+	1713	Protein_4494	flagellar biosynthesis protein FlhA	-
LSI73_RS22930 (4488165)	4488165..4488662	-	498	Protein_4495	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T227967 WP_001263495.1 NZ_CP089272:c4484077-4483679 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T227967 NZ_CP123240:4706574-4706951 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT227967 WP_000554757.1 NZ_CP089272:c4484373-4484080 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT227967 NZ_CP123240:4706153-4706527 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6