Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 3334276..3335071 | Replicon | chromosome |
| Accession | NZ_CP089272 | ||
| Organism | Escherichia coli O157:H7 strain M1300706001A | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | B7UP43 |
| Locus tag | LSI73_RS17350 | Protein ID | WP_000854914.1 |
| Coordinates | 3334276..3334650 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A0B0VS41 |
| Locus tag | LSI73_RS17355 | Protein ID | WP_001280954.1 |
| Coordinates | 3334697..3335071 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LSI73_RS17310 (3329280) | 3329280..3329771 | - | 492 | WP_001301587.1 | DUF1097 domain-containing protein | - |
| LSI73_RS17315 (3329873) | 3329873..3330427 | - | 555 | WP_001001917.1 | molecular chaperone YcdY | - |
| LSI73_RS17320 (3330451) | 3330451..3331188 | - | 738 | WP_000283664.1 | zinc-binding phosphatase | - |
| LSI73_RS17325 (3331243) | 3331243..3332181 | - | 939 | WP_000351284.1 | glyoxylate/hydroxypyruvate reductase GhrA | - |
| LSI73_RS17335 (3332652) | 3332652..3333494 | - | 843 | WP_001280481.1 | DUF4942 domain-containing protein | - |
| LSI73_RS17340 (3333579) | 3333579..3333776 | - | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| LSI73_RS17345 (3333788) | 3333788..3334279 | - | 492 | WP_000976853.1 | DUF5983 family protein | - |
| LSI73_RS17350 (3334276) | 3334276..3334650 | - | 375 | WP_000854914.1 | TA system toxin CbtA family protein | Toxin |
| LSI73_RS17355 (3334697) | 3334697..3335071 | - | 375 | WP_001280954.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| LSI73_RS17360 (3335234) | 3335234..3335455 | - | 222 | WP_001220314.1 | DUF987 domain-containing protein | - |
| LSI73_RS17365 (3335518) | 3335518..3335994 | - | 477 | WP_001186738.1 | RadC family protein | - |
| LSI73_RS17370 (3336010) | 3336010..3336495 | - | 486 | WP_000214398.1 | antirestriction protein | - |
| LSI73_RS17375 (3336586) | 3336586..3337404 | - | 819 | WP_001234682.1 | DUF932 domain-containing protein | - |
| LSI73_RS17380 (3337744) | 3337744..3338814 | - | 1071 | WP_000102669.1 | patatin-like phospholipase family protein | - |
| LSI73_RS17385 (3338811) | 3338811..3339716 | - | 906 | WP_000203541.1 | diguanylate cyclase regulator RdcB family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Genomic island | - | csgB / csgD / csgE / csgF / csgG | 3325662..3336495 | 10833 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14117.17 Da Isoelectric Point: 7.7761
>T227961 WP_000854914.1 NZ_CP089272:c3334650-3334276 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T227961 NZ_CP123240:3755212-3755430 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13718.51 Da Isoelectric Point: 6.6249
>AT227961 WP_001280954.1 NZ_CP089272:c3335071-3334697 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT227961 NZ_CP123240:3754812-3755186 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9LXR5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0B0VS41 |