Detailed information of TA system TA227958

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2677350..2677575
• Replicon: chromosome
• Accession: NZ_CP089272
• Organism: Escherichia coli O157:H7 strain M1300706001A

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: LSI73_RS13790
• Protein ID: WP_000813258.1
• Coordinates: 2677420..2677575 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2677350..2677408 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSI73_RS13740	2672415..2672657	+	243	WP_000747948.1	Cro/CI family transcriptional regulator	-
LSI73_RS13745	2672641..2673066	+	426	WP_000693878.1	toxin YdaT family protein	-
LSI73_RS13750	2673135..2674178	+	1044	WP_001262402.1	DnaT-like ssDNA-binding domain-containing protein	-
LSI73_RS13755	2674171..2674632	+	462	WP_000139447.1	replication protein P	-
LSI73_RS13760	2674666..2675382	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
LSI73_RS13765	2675415..2675696	+	282	WP_000603384.1	DNA-binding protein	-
LSI73_RS13770	2675693..2675920	+	228	WP_000699809.1	hypothetical protein	-
LSI73_RS13775	2675913..2676224	+	312	WP_001289673.1	hypothetical protein	-
LSI73_RS13780	2676352..2676570	+	219	WP_000683609.1	DUF4014 family protein	-
LSI73_RS13785	2676572..2677129	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2677350..2677408	-	59	-	-	Antitoxin
LSI73_RS13790	2677420..2677575	+	156	WP_000813258.1	Hok/Gef family protein	Toxin
LSI73_RS13795	2677695..2678039	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
LSI73_RS13800	2678161..2678433	+	273	WP_000191872.1	hypothetical protein	-
LSI73_RS13805	2678435..2679484	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
LSI73_RS13810	2679497..2679802	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
LSI73_RS13815	2679865..2680419	+	555	WP_000640035.1	DUF1133 family protein	-
LSI73_RS13820	2680644..2680841	+	198	WP_000917763.1	hypothetical protein	-
LSI73_RS13825	2680977..2681690	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
LSI73_RS13840	2682141..2682572	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2608963..2709817	100854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T227958 WP_000813258.1 NZ_CP089272:2677420-2677575 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T227958 NZ_CP123240:2914526-2914633 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT227958 NZ_CP089272:c2677408-2677350 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin