Detailed information of TA system TA227933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4615356..4616050
• Replicon: chromosome
• Accession: NZ_CP089270
• Organism: Escherichia coli O157:H7 strain M1300706002

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: LSI72_RS23765
• Protein ID: WP_001263495.1
• Coordinates: 4615356..4615754 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: LSI72_RS23770
• Protein ID: WP_000554757.1
• Coordinates: 4615757..4616050 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4610943)	4610943..4611023	-	81	NuclAT_12	-	-
- (4610943)	4610943..4611023	-	81	NuclAT_12	-	-
- (4610943)	4610943..4611023	-	81	NuclAT_12	-	-
- (4610943)	4610943..4611023	-	81	NuclAT_12	-	-
LSI72_RS23740 (4611619)	4611619..4612077	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LSI72_RS23745 (4612338)	4612338..4613795	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
LSI72_RS23750 (4613852)	4613852..4614373	-	522	Protein_4659	peptide chain release factor H	-
LSI72_RS23755 (4614372)	4614372..4614575	-	204	Protein_4660	RtcB family protein	-
LSI72_RS23760 (4614894)	4614894..4615346	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
LSI72_RS23765 (4615356)	4615356..4615754	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LSI72_RS23770 (4615757)	4615757..4616050	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LSI72_RS23775 (4616102)	4616102..4617157	-	1056	WP_001226188.1	DNA polymerase IV	-
LSI72_RS23780 (4617228)	4617228..4618013	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
LSI72_RS23785 (4617985)	4617985..4619697	+	1713	Protein_4666	flagellar biosynthesis protein FlhA	-
LSI72_RS23790 (4619842)	4619842..4620339	-	498	Protein_4667	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T227933 WP_001263495.1 NZ_CP089270:c4615754-4615356 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T227933 NZ_CP123237:2329741-2330028 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT227933 WP_000554757.1 NZ_CP089270:c4616050-4615757 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT227933 NZ_CP123237:2329502-2329741 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6