Detailed information of TA system TA227932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4594729..4595423
• Replicon: chromosome
• Accession: NZ_CP089270
• Organism: Escherichia coli O157:H7 strain M1300706002

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: LSI72_RS23625
• Protein ID: WP_001263495.1
• Coordinates: 4594729..4595127 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: LSI72_RS23630
• Protein ID: WP_000554757.1
• Coordinates: 4595130..4595423 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4590317)	4590317..4590397	-	81	NuclAT_13	-	-
- (4590317)	4590317..4590397	-	81	NuclAT_13	-	-
- (4590317)	4590317..4590397	-	81	NuclAT_13	-	-
- (4590317)	4590317..4590397	-	81	NuclAT_13	-	-
LSI72_RS23600 (4590993)	4590993..4591451	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LSI72_RS23605 (4591712)	4591712..4593169	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
LSI72_RS23610 (4593226)	4593226..4593747	-	522	Protein_4632	peptide chain release factor H	-
LSI72_RS23615 (4593746)	4593746..4593949	-	204	Protein_4633	RtcB family protein	-
LSI72_RS23620 (4594268)	4594268..4594719	-	452	Protein_4634	GNAT family N-acetyltransferase	-
LSI72_RS23625 (4594729)	4594729..4595127	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LSI72_RS23630 (4595130)	4595130..4595423	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LSI72_RS23635 (4595483)	4595483..4596527	-	1045	Protein_4637	DNA polymerase IV	-
LSI72_RS23640 (4596598)	4596598..4597379	-	782	Protein_4638	putative lateral flagellar export/assembly protein LafU	-
LSI72_RS23645 (4597351)	4597351..4599062	+	1712	Protein_4639	flagellar biosynthesis protein FlhA	-
LSI72_RS23650 (4599205)	4599205..4599608	-	404	Protein_4640	transposase	-
LSI72_RS23655 (4599615)	4599615..4599968	+	354	Protein_4641	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T227932 WP_001263495.1 NZ_CP089270:c4595127-4594729 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T227932 NZ_CP123237:2048148-2048251 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT227932 WP_000554757.1 NZ_CP089270:c4595423-4595130 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT227932 NZ_CP123237:c2048289-2048018 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: S1FJN6