Detailed information of TA system TA227910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5376383..5376978
• Replicon: chromosome
• Accession: NZ_CP089238
• Organism: Pseudomonas aeruginosa strain JNQH-PA033

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: LSG45_RS24895
• Protein ID: WP_003113526.1
• Coordinates: 5376700..5376978 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSG45_RS24890
• Protein ID: WP_003113527.1
• Coordinates: 5376383..5376688 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG45_RS24870 (LSG45_24870)	5371972..5373030	+	1059	WP_033969891.1	hypothetical protein	-
LSG45_RS24875 (LSG45_24875)	5373085..5373948	-	864	WP_234575032.1	integrase domain-containing protein	-
LSG45_RS24880 (LSG45_24880)	5374550..5375692	-	1143	WP_234575034.1	STY4528 family pathogenicity island replication protein	-
LSG45_RS24890 (LSG45_24890)	5376383..5376688	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
LSG45_RS24895 (LSG45_24895)	5376700..5376978	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSG45_RS24900 (LSG45_24900)	5377031..5377159	-	129	Protein_4923	integrase	-
LSG45_RS24905 (LSG45_24905)	5377307..5379535	+	2229	WP_021264080.1	TonB-dependent receptor	-
LSG45_RS24910 (LSG45_24910)	5379605..5380252	-	648	WP_003095021.1	carbonate dehydratase	-
LSG45_RS24915 (LSG45_24915)	5380314..5381552	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T227910 WP_003113526.1 NZ_CP089238:c5376978-5376700 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T227910 NZ_CP123231:3535666-3535884 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT227910 WP_003113527.1 NZ_CP089238:c5376688-5376383 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT227910 NZ_CP123231:3535266-3535640 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: V6ALY3