Detailed information of TA system TA227902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5787613..5788208
• Replicon: chromosome
• Accession: NZ_CP089236
• Organism: Pseudomonas aeruginosa strain JNQH-PA027

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: LSG40_RS28140
• Protein ID: WP_003113526.1
• Coordinates: 5787930..5788208 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSG40_RS28135
• Protein ID: WP_003113527.1
• Coordinates: 5787613..5787918 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG40_RS28115 (LSG40_28115)	5783689..5783946	+	258	WP_010793759.1	ATP-binding protein	-
LSG40_RS28120 (LSG40_28120)	5784322..5785185	-	864	WP_010793758.1	integrase domain-containing protein	-
LSG40_RS28125 (LSG40_28125)	5785787..5786929	-	1143	WP_010793757.1	STY4528 family pathogenicity island replication protein	-
LSG40_RS28135 (LSG40_28135)	5787613..5787918	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
LSG40_RS28140 (LSG40_28140)	5787930..5788208	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSG40_RS28145 (LSG40_28145)	5788261..5788389	-	129	Protein_5581	integrase	-
LSG40_RS28150 (LSG40_28150)	5788537..5790765	+	2229	WP_003099265.1	TonB-dependent receptor	-
LSG40_RS28155 (LSG40_28155)	5790835..5791482	-	648	WP_003095021.1	carbonate dehydratase	-
LSG40_RS28160 (LSG40_28160)	5791544..5792782	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T227902 WP_003113526.1 NZ_CP089236:c5788208-5787930 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T227902 NZ_CP123231:974514-974921 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT227902 WP_003113527.1 NZ_CP089236:c5787918-5787613 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT227902 NZ_CP123231:974267-974533 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATTCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V6ALY3