Detailed information of TA system TA227894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 160922..161649
• Replicon: plasmid pK54798
• Accession: NZ_CP089235
• Organism: Klebsiella sp. KPN54798

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LPQ25_RS26880
• Protein ID: WP_011251285.1
• Coordinates: 160922..161233 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LPQ25_RS26885
• Protein ID: WP_011251286.1
• Coordinates: 161230..161649 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPQ25_RS26835 (LPQ25_26835)	155986..156501	-	516	WP_041937820.1	hypothetical protein	-
LPQ25_RS26840 (LPQ25_26840)	156609..157208	-	600	WP_234599521.1	hypothetical protein	-
LPQ25_RS26845 (LPQ25_26845)	157299..157616	-	318	WP_228263089.1	hypothetical protein	-
LPQ25_RS26850 (LPQ25_26850)	157613..158254	-	642	WP_234599522.1	DsbA family protein	-
LPQ25_RS26855 (LPQ25_26855)	158314..158655	-	342	WP_011251281.1	hypothetical protein	-
LPQ25_RS26860 (LPQ25_26860)	158669..158980	-	312	WP_011251282.1	hypothetical protein	-
LPQ25_RS26865 (LPQ25_26865)	158997..159446	-	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
LPQ25_RS26875 (LPQ25_26875)	160280..160717	+	438	Protein_182	DDE-type integrase/transposase/recombinase	-
LPQ25_RS26880 (LPQ25_26880)	160922..161233	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LPQ25_RS26885 (LPQ25_26885)	161230..161649	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LPQ25_RS26890 (LPQ25_26890)	161796..162763	+	968	Protein_185	IS5 family transposase	-
LPQ25_RS26895 (LPQ25_26895)	162835..163200	-	366	WP_048333448.1	hypothetical protein	-
LPQ25_RS26900 (LPQ25_26900)	163214..164002	-	789	WP_040217257.1	hypothetical protein	-
LPQ25_RS26905 (LPQ25_26905)	164023..164643	-	621	WP_234599523.1	conjugative transfer protein MobI(A/C)	-
LPQ25_RS26910 (LPQ25_26910)	165062..165697	+	636	WP_223171879.1	hypothetical protein	-
LPQ25_RS26915 (LPQ25_26915)	166010..166480	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN / iroN / iroD / iroC / iroB	1..226890	226890
	-	inside	IScluster/Tn	-	-	159448..171769	12321

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T227894 WP_011251285.1 NZ_CP089235:160922-161233 [Klebsiella sp. KPN54798]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T227894 NZ_CP123227:11762-12034 [Pseudomonas amygdali pv. aesculi]
GTGCTAGTTGAGTGGCTTCCACAAGCTTTGGAGGCACTGGAGGAAATCGCCGGCTACCTAGCCGAGCGCAACCCTTATGC
TGCCGAGCAAATGCAGGCCACCCTTGAGGCCACGGTCGAGGCTCTCCCCCTGCACCCTTACATCCACCGTCCCGGCCGTG
TTCCAGGAACGCGCGAAGCGGTTGTGCATCCCAACTACCTGGTTGTCTACCGCGTCGGTACAGAGCGCATCAGCATCGTG
GACGTCCTGCACGCCCGCCAAGAGTACCCCTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT227894 WP_011251286.1 NZ_CP089235:161230-161649 [Klebsiella sp. KPN54798]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT227894 NZ_CP123227:11587-11772 [Pseudomonas amygdali pv. aesculi]
ATGAGCATTCAACTTGACCCTCGCGTCTCGGAGTTCGAGACCCAGGAACAGGCCGACAATTACGACCGTTGGTTTCGCCT
GCGTATCGAGCGCAGTTTGGCCGATCCGCGTCCACCCGTGCCGCACGATGAAGCAATGGCTCGCGTCAGAGCCATGATTG
AGGCAAAGCGCCGCCGTGCTAGTTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1