Detailed information of TA system TA227856

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): TglT-TakA/AbiEii-AbiEi
• Location: 1463856..1465318
• Replicon: chromosome
• Accession: NZ_CP089224
• Organism: Mycobacterium ostraviense strain FDAARGOS_1613

Toxin (Protein)

• Gene name: tglT
• Uniprot ID: A0A163SZJ8
• Locus tag: LTS72_RS06700
• Protein ID: WP_075513619.1
• Coordinates: 1463856..1464737 (-)
• Length: 294 a.a.

Antitoxin (Protein)

• Gene name: takA
• Uniprot ID: -
• Locus tag: LTS72_RS06705
• Protein ID: WP_082275213.1
• Coordinates: 1464734..1465318 (-)
• Length: 195 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS72_RS06680 (LTS72_06680)	1459410..1460654	+	1245	WP_075509744.1	IS701 family transposase	-
LTS72_RS06685 (LTS72_06685)	1460769..1461524	+	756	WP_075513616.1	DUF6430 domain-containing protein	-
LTS72_RS06690 (LTS72_06690)	1461521..1461970	+	450	WP_082277435.1	HIT family protein	-
LTS72_RS06695 (LTS72_06695)	1462312..1463436	+	1125	WP_075513618.1	hypothetical protein	-
LTS72_RS06700 (LTS72_06700)	1463856..1464737	-	882	WP_075513619.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LTS72_RS06705 (LTS72_06705)	1464734..1465318	-	585	WP_082275213.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
LTS72_RS06710 (LTS72_06710)	1465602..1465910	+	309	WP_233026998.1	hypothetical protein	-
LTS72_RS06715 (LTS72_06715)	1465907..1466509	+	603	WP_075513622.1	hypothetical protein	-
LTS72_RS06720 (LTS72_06720)	1467885..1468397	+	513	WP_233026999.1	hypothetical protein	-
LTS72_RS06725 (LTS72_06725)	1468622..1468933	+	312	WP_075513628.1	hypothetical protein	-
LTS72_RS06730 (LTS72_06730)	1469426..1469989	+	564	WP_075513629.1	HD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1457575..1478083	20508

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (49-249)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (5-53)

Sequences

Toxin

Protein

• Length: 294 a.a.
• Molecular weight: 31830.33 Da
• Isoelectric Point: 5.3731

>T227856 WP_075513619.1 NZ_CP089224:c1464737-1463856 [Mycobacterium ostraviense]
VTKPYSSPPTNLRSLRDRLTQAAQRQGVVFGRLQRHIAMIVVAQFAAMLTDDSGAPLLLVKGGSSLELRRGIPDSRTSKD
FDTVARRDIATVHEQLADAGETGWEGFTATFTVPEEIDVPGMPVKPRRFTAKLSYRGQPFVSVPIEVSNVEAGNADQFDT
LTSDALGLVGVPAAVAVPCMTIPWQIAQKLHAVTAVLEAPRVNDRAHDLVDLQLLEGLLPDSDLLPTRSACIAVFEARAQ
HPWPPQVTALPHWPPIYSGALEGLDHLELAATVEEAVKAVRRFVERIDVATET

Nucleotide

• Length: 882 bp

>T227856 NZ_CP123197:2163320-2163427 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGTGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 195 a.a.
• Molecular weight: 21202.13 Da
• Isoelectric Point: 10.2756

>AT227856 WP_082275213.1 NZ_CP089224:c1465318-1464734 [Mycobacterium ostraviense]
MTIWDRLVELAAEQHGYVTTRDARDIGVDPVQLRLLAARGRLERAGRGVYRVPVLPRGEHDDLAAAVSWTLGRGVISHES
ALGLHALSDVNPSRIHLTVPRENHPRAAGGELYRLHRRDLQATDITLVDGIPVTTAVRTIRDCLKAGTDPYQLRVAIERA
EAEGTLRRAPAAELRAALDQPAAGVRARTKRASA

Nucleotide

• Length: 585 bp

>AT227856 NZ_CP123197:c2163267-2163206 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A163SZJ8