Detailed information of TA system TA227831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 963211..963884
• Replicon: chromosome
• Accession: NZ_CP089218
• Organism: Mycobacterium kansasii strain FDAARGOS_1614

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A653F4R7
• Locus tag: LTT71_RS04340
• Protein ID: WP_036391761.1
• Coordinates: 963537..963884 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LTT71_RS04335
• Protein ID: WP_023371783.1
• Coordinates: 963211..963540 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT71_RS04315 (LTT71_04315)	959194..960399	-	1206	WP_023371775.1	RtcB family protein	-
LTT71_RS04320 (LTT71_04320)	960565..960759	-	195	WP_129111635.1	hypothetical protein	-
LTT71_RS04330 (LTT71_04330)	961412..963052	+	1641	WP_080674111.1	AAA family ATPase	-
LTT71_RS04335 (LTT71_04335)	963211..963540	-	330	WP_023371783.1	XRE family transcriptional regulator	Antitoxin
LTT71_RS04340 (LTT71_04340)	963537..963884	-	348	WP_036391761.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LTT71_RS04345 (LTT71_04345)	963937..964125	-	189	WP_023371787.1	hypothetical protein	-
LTT71_RS04350 (LTT71_04350)	964179..966278	-	2100	WP_023371789.1	hypothetical protein	-
LTT71_RS04355 (LTT71_04355)	966285..966611	-	327	WP_023371791.1	helix-turn-helix transcriptional regulator	-
LTT71_RS04360 (LTT71_04360)	966608..967687	-	1080	WP_023371793.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	956147..973803	17656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (35-97)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13049.82 Da
• Isoelectric Point: 4.4057

>T227831 WP_036391761.1 NZ_CP089218:c963884-963537 [Mycobacterium kansasii]
MSWDVILLEEVEAWYFSLDDEAMAAVTGAIDLLELEGPTLGRPTVDKVKGSKFHSLKELRPAGTSIRILFIFDPRRQAIL
LLGGDKAGNWKRWYEENIPVADQRYENWLANEGGE

Nucleotide

• Length: 348 bp

>T227831 NZ_CP123047:c83261-82863 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11701.20 Da
• Isoelectric Point: 7.4048

>AT227831 WP_023371783.1 NZ_CP089218:c963540-963211 [Mycobacterium kansasii]
MIVARNWREIRAEAVAQGRLDPARADAARKEMHDAVQAQRLADIRKAHGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQSYVAALGGNLRIVADFGESSVELTA

Nucleotide

• Length: 330 bp

>AT227831 NZ_CP123047:c83488-83261 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A653F4R7