Detailed information of TA system TA022783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 184346..184961
• Replicon: chromosome
• Accession: NC_010552
• Organism: Burkholderia ambifaria MC40-6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B1YYE7
• Locus tag: BAMMC406_RS16555
• Protein ID: WP_012365190.1
• Coordinates: 184620..184961 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: B1YYE6
• Locus tag: BAMMC406_RS16550
• Protein ID: WP_012365189.1
• Coordinates: 184346..184633 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAMMC406_RS16515	179391..179858	+	468	WP_012365184.1	cupin domain-containing protein	-
BAMMC406_RS16520	179928..180749	+	822	WP_012365185.1	SDR family oxidoreductase	-
BAMMC406_RS16525	180855..181634	-	780	WP_012365186.1	5'/3'-nucleotidase SurE	-
BAMMC406_RS16530	181824..182123	-	300	WP_034177128.1	AzlD family protein	-
BAMMC406_RS16535	182116..182853	-	738	WP_012365187.1	AzlC family ABC transporter permease	-
BAMMC406_RS16540	182979..183437	+	459	WP_012365188.1	Lrp/AsnC family transcriptional regulator	-
BAMMC406_RS16545	183653..184222	+	570	WP_006749371.1	hypothetical protein	-
BAMMC406_RS16550	184346..184633	-	288	WP_012365189.1	helix-turn-helix domain-containing protein	Antitoxin
BAMMC406_RS16555	184620..184961	-	342	WP_012365190.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BAMMC406_RS16560	185177..185809	-	633	WP_012365191.1	LysE family translocator	-
BAMMC406_RS16565	186241..187878	+	1638	WP_175774928.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (21-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-87)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13153.39 Da
• Isoelectric Point: 10.1617

>T22783 WP_012365190.1 NC_010552:c184961-184620 [Burkholderia ambifaria MC40-6]
MRTVAYEIHFFSEQVEAGVFALPKTLTARFFAIADRMEQYGPNLGEPHTRPMGDGLYEMRLKGLEGIARVFYCAAVDRRI
VMLHCFVKKTQKTPRKELETARRRLKEIRYGNL

Nucleotide

• Length: 342 bp

>T22783 NC_010552:c184961-184620 [Burkholderia ambifaria MC40-6]
ATGCGAACCGTCGCGTATGAAATCCATTTCTTCAGCGAGCAAGTCGAGGCAGGCGTGTTTGCGTTGCCGAAGACGCTGAC
CGCCAGGTTCTTCGCAATCGCTGACCGTATGGAGCAGTACGGCCCGAATCTGGGCGAACCTCACACCAGGCCGATGGGCG
ACGGGTTGTATGAAATGCGCTTGAAGGGCCTGGAAGGTATCGCCCGTGTGTTTTACTGTGCGGCCGTCGACCGGCGGATC
GTCATGCTTCACTGCTTCGTGAAGAAGACGCAGAAAACGCCGCGAAAAGAACTGGAAACCGCGCGCCGCCGATTGAAGGA
GATTCGATATGGCAACCTATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10421.07 Da
• Isoelectric Point: 10.6526

>AT22783 WP_012365189.1 NC_010552:c184633-184346 [Burkholderia ambifaria MC40-6]
MATYKALRERALSDPKVRAEYDRLNREEFALLDAMLAARRAAGLSQADVAERMGTKAPAVTRLERALATGRHSPSIDTLR
KYAAACGKKLVISFA

Nucleotide

• Length: 288 bp

>AT22783 NC_010552:c184633-184346 [Burkholderia ambifaria MC40-6]
ATGGCAACCTATAAGGCACTGCGCGAGCGCGCATTGTCCGATCCGAAGGTACGCGCCGAATACGATCGGCTGAACCGCGA
GGAATTCGCGCTGCTCGACGCAATGCTGGCCGCCCGGCGAGCCGCCGGTCTTTCGCAGGCCGATGTTGCGGAGCGCATGG
GCACGAAGGCGCCTGCCGTCACCCGGCTCGAACGCGCGCTCGCGACAGGCCGGCACTCTCCATCGATCGACACGCTGCGC
AAGTATGCCGCCGCGTGCGGCAAGAAACTCGTGATCTCTTTCGCCTGA

Structures

Toxin

• AlphaFold DB: B1YYE7

Antitoxin

• AlphaFold DB: B1YYE6