Detailed information of TA system TA227821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2422752..2423425
• Replicon: chromosome
• Accession: NZ_CP089216
• Organism: Mycobacterium kansasii strain FDAARGOS_1615

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A653F4R7
• Locus tag: LTS70_RS10945
• Protein ID: WP_036391761.1
• Coordinates: 2423078..2423425 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LTS70_RS10940
• Protein ID: WP_023371783.1
• Coordinates: 2422752..2423081 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS70_RS10920 (LTS70_10920)	2418735..2419940	-	1206	WP_023371775.1	RtcB family protein	-
LTS70_RS10925 (LTS70_10925)	2420106..2420300	-	195	WP_129111635.1	hypothetical protein	-
LTS70_RS10935 (LTS70_10935)	2420953..2422593	+	1641	WP_080674111.1	AAA family ATPase	-
LTS70_RS10940 (LTS70_10940)	2422752..2423081	-	330	WP_023371783.1	XRE family transcriptional regulator	Antitoxin
LTS70_RS10945 (LTS70_10945)	2423078..2423425	-	348	WP_036391761.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LTS70_RS10950 (LTS70_10950)	2423478..2423666	-	189	WP_023371787.1	hypothetical protein	-
LTS70_RS10955 (LTS70_10955)	2423720..2425819	-	2100	WP_023371789.1	hypothetical protein	-
LTS70_RS10960 (LTS70_10960)	2425826..2426152	-	327	WP_023371791.1	helix-turn-helix transcriptional regulator	-
LTS70_RS10965 (LTS70_10965)	2426149..2427228	-	1080	WP_023371793.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2406209..2433344	27135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (35-97)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13049.82 Da
• Isoelectric Point: 4.4057

>T227821 WP_036391761.1 NZ_CP089216:c2423425-2423078 [Mycobacterium kansasii]
MSWDVILLEEVEAWYFSLDDEAMAAVTGAIDLLELEGPTLGRPTVDKVKGSKFHSLKELRPAGTSIRILFIFDPRRQAIL
LLGGDKAGNWKRWYEENIPVADQRYENWLANEGGE

Nucleotide

• Length: 348 bp

>T227821 NZ_CP123046:3521836-3522054 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11701.20 Da
• Isoelectric Point: 7.4048

>AT227821 WP_023371783.1 NZ_CP089216:c2423081-2422752 [Mycobacterium kansasii]
MIVARNWREIRAEAVAQGRLDPARADAARKEMHDAVQAQRLADIRKAHGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQSYVAALGGNLRIVADFGESSVELTA

Nucleotide

• Length: 330 bp

>AT227821 NZ_CP123046:3521436-3521810 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A653F4R7