Detailed information of TA system TA227805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 1610816..1611324
• Replicon: chromosome
• Accession: NZ_CP089211
• Organism: Sinomicrobium kalidii strain HD2P242

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LS482_RS06465
• Protein ID: WP_233030936.1
• Coordinates: 1611064..1611324 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LS482_RS06460
• Protein ID: WP_233030935.1
• Coordinates: 1610816..1611064 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LS482_RS06425 (LS482_06425)	1606409..1607569	-	1161	WP_233030928.1	efflux RND transporter periplasmic adaptor subunit	-
LS482_RS06430 (LS482_06430)	1607857..1608423	-	567	WP_233030929.1	RNA polymerase sigma-70 factor	-
LS482_RS06435 (LS482_06435)	1608524..1608754	-	231	WP_233030930.1	hypothetical protein	-
LS482_RS06440 (LS482_06440)	1608946..1609149	+	204	WP_233030931.1	helix-turn-helix transcriptional regulator	-
LS482_RS06445 (LS482_06445)	1609350..1609658	+	309	WP_233030932.1	hypothetical protein	-
LS482_RS06450 (LS482_06450)	1609672..1610061	+	390	WP_233030933.1	putative toxin-antitoxin system toxin component, PIN family	-
LS482_RS06455 (LS482_06455)	1610248..1610490	+	243	WP_233030934.1	hypothetical protein	-
LS482_RS06460 (LS482_06460)	1610816..1611064	+	249	WP_233030935.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LS482_RS06465 (LS482_06465)	1611064..1611324	+	261	WP_233030936.1	Txe/YoeB family addiction module toxin	Toxin
LS482_RS06470 (LS482_06470)	1611638..1611949	-	312	WP_233030937.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LS482_RS06475 (LS482_06475)	1611952..1612173	-	222	WP_233030938.1	hypothetical protein	-
LS482_RS06480 (LS482_06480)	1612326..1612598	+	273	WP_233030939.1	hypothetical protein	-
LS482_RS06485 (LS482_06485)	1612570..1613040	-	471	WP_233030940.1	hypothetical protein	-
LS482_RS06490 (LS482_06490)	1613705..1613998	+	294	WP_233030941.1	hypothetical protein	-
LS482_RS06495 (LS482_06495)	1614097..1615239	-	1143	WP_233030942.1	acyl-CoA dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10277.90 Da
• Isoelectric Point: 9.6012

>T227805 WP_233030936.1 NZ_CP089211:1611064-1611324 [Sinomicrobium kalidii]
MRLIWSTTSSNDYLYCQKVDKKIVKRINELIKNCMRTPFEGIGKPEALKGGLQGYWSRRITSEHRLVYKYEDEELWIAAC
RYHYGK

Nucleotide

• Length: 261 bp

>T227805 NZ_CP123043:c4876547-4876263 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9471.88 Da
• Isoelectric Point: 5.8559

>AT227805 WP_233030935.1 NZ_CP089211:1610816-1611064 [Sinomicrobium kalidii]
METVNYTDFRANLKHWFDKVINDVSDIVIKRKGGKDLVLISLDEYNSLKETTYLLTGKNRDVLLKSIKELEGDKGVQKDL
IE

Nucleotide

• Length: 249 bp

>AT227805 NZ_CP123043:c4876779-4876537 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA