Detailed information of TA system TA227761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 350096..350613
• Replicon: chromosome
• Accession: NZ_CP089161
• Organism: Staphylococcus aureus strain UNC_SaCF13

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: LTK21_RS01815
• Protein ID: WP_000113265.1
• Coordinates: 350096..350362 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: LTK21_RS01820
• Protein ID: WP_000584499.1
• Coordinates: 350362..350613 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTK21_RS01785 (LTK21_01785)	345524..346060	-	537	WP_165624904.1	GNAT family N-acetyltransferase	-
LTK21_RS01790 (LTK21_01790)	346294..347118	-	825	WP_000572038.1	formate/nitrite transporter family protein	-
LTK21_RS01795 (LTK21_01795)	347335..347517	-	183	WP_000230292.1	hypothetical protein	-
LTK21_RS01800 (LTK21_01800)	347601..348068	-	468	WP_000153347.1	SRPBCC domain-containing protein	-
LTK21_RS01805 (LTK21_01805)	348254..349801	-	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
LTK21_RS01810 (LTK21_01810)	349990..350061	-	72	Protein_348	hypothetical protein	-
LTK21_RS01815 (LTK21_01815)	350096..350362	-	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
LTK21_RS01820 (LTK21_01820)	350362..350613	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LTK21_RS01825 (LTK21_01825)	350778..350858	-	81	WP_223197974.1	hypothetical protein	-
LTK21_RS01830 (LTK21_01830)	350886..351485	-	600	WP_000162810.1	DsbA family protein	-
LTK21_RS01835 (LTK21_01835)	351504..351866	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
LTK21_RS01840 (LTK21_01840)	352121..353371	+	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
LTK21_RS01845 (LTK21_01845)	353468..354199	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
LTK21_RS01850 (LTK21_01850)	354196..354915	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T227761 WP_000113265.1 NZ_CP089161:c350362-350096 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T227761 NZ_CP123031:69423-69839 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT227761 WP_000584499.1 NZ_CP089161:c350613-350362 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT227761 NZ_CP123031:69196-69426 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60