Detailed information of TA system TA227749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2511010..2511533
• Replicon: chromosome
• Accession: NZ_CP089159
• Organism: Staphylococcus aureus strain UNC_SaCF18

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: X5DYX7
• Locus tag: LTK20_RS12690
• Protein ID: WP_000074048.1
• Coordinates: 2511267..2511533 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: W8TQT5
• Locus tag: LTK20_RS12685
• Protein ID: WP_000587616.1
• Coordinates: 2511010..2511267 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTK20_RS12660 (2506090)	2506090..2507283	+	1194	WP_000675401.1	MFS transporter	-
LTK20_RS12665 (2507507)	2507507..2508256	-	750	WP_000072444.1	CPBP family lipoprotein N-acylation protein LnsB	-
LTK20_RS12670 (2508451)	2508451..2510028	+	1578	WP_000143503.1	FMN-binding glutamate synthase family protein	-
LTK20_RS12675 (2510196)	2510196..2510651	-	456	WP_000732082.1	DUF1307 domain-containing protein	-
LTK20_RS12680 (2510710)	2510710..2510904	+	195	WP_001790145.1	hypothetical protein	-
LTK20_RS12685 (2511010)	2511010..2511267	+	258	WP_000587616.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LTK20_RS12690 (2511267)	2511267..2511533	+	267	WP_000074048.1	Txe/YoeB family addiction module toxin	Toxin
LTK20_RS12695 (2511888)	2511888..2512964	+	1077	WP_001076671.1	M42 family metallopeptidase	-
LTK20_RS12700 (2513212)	2513212..2513874	+	663	WP_000923520.1	ATP-binding cassette domain-containing protein	-
LTK20_RS12705 (2513867)	2513867..2514643	+	777	WP_000072147.1	iron export ABC transporter permease subunit FetB	-
LTK20_RS12710 (2515039)	2515039..2516226	+	1188	WP_000026194.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (25-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10648.23 Da
• Isoelectric Point: 10.1384

>T227749 WP_000074048.1 NZ_CP089159:2511267-2511533 [Staphylococcus aureus]
MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Nucleotide

• Length: 267 bp

>T227749 NZ_CP123029:c1847637-1847263 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9774.73 Da
• Isoelectric Point: 4.1353

>AT227749 WP_000587616.1 NZ_CP089159:2511010-2511267 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT227749 NZ_CP123029:c1848095-1847727 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• PDB: 7V6W
• PDB: 7V5Y
• PDB: 7V5Z

Antitoxin

• AlphaFold DB: W8TQT5