Detailed information of TA system TA227745

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): TMCS/-
• Location: 622922..623501
• Replicon: chromosome
• Accession: NZ_CP089159
• Organism: Staphylococcus aureus strain UNC_SaCF18

Toxin (Protein)

• Gene name: MW1434
• Uniprot ID: A0A4P7P609
• Locus tag: LTK20_RS03540
• Protein ID: WP_001025401.1
• Coordinates: 622922..623167 (+)
• Length: 82 a.a.

Antitoxin (RNA)

Gene name	MW1433
Locus tag	-
Coordinates	623136..623501 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTK20_RS03490 (619173)	619173..619787	+	615	WP_000191466.1	hypothetical protein	-
LTK20_RS03495 (619784)	619784..619930	-	147	WP_001795334.1	hypothetical protein	-
LTK20_RS03500 (619966)	619966..620148	-	183	WP_000705240.1	hypothetical protein	-
LTK20_RS03505 (620218)	620218..620949	-	732	WP_000511090.1	XRE family transcriptional regulator	-
LTK20_RS03510 (621101)	621101..621313	+	213	WP_000213811.1	helix-turn-helix transcriptional regulator	-
LTK20_RS03515 (621361)	621361..621804	+	444	WP_000435357.1	hypothetical protein	-
LTK20_RS03520 (621819)	621819..621968	+	150	WP_000771849.1	hypothetical protein	-
LTK20_RS03525 (622009)	622009..622221	-	213	WP_000362644.1	hypothetical protein	-
LTK20_RS03530 (622291)	622291..622488	+	198	WP_001148860.1	hypothetical protein	-
LTK20_RS03535 (622475)	622475..622855	-	381	WP_000773059.1	DUF2513 domain-containing protein	-
LTK20_RS03540 (622922)	622922..623167	+	246	WP_001025401.1	DUF2829 domain-containing protein	Toxin
LTK20_RS03545 (623136)	623136..623501	-	366	WP_001128433.1	hypothetical protein	Antitoxin
LTK20_RS03550 (623556)	623556..623771	+	216	WP_001097552.1	hypothetical protein	-
LTK20_RS03555 (623796)	623796..624059	+	264	WP_001124160.1	helix-turn-helix domain-containing protein	-
LTK20_RS03560 (624072)	624072..624233	+	162	WP_001285954.1	DUF1270 domain-containing protein	-
LTK20_RS03565 (624312)	624312..624635	+	324	WP_000174998.1	hypothetical protein	-
LTK20_RS03570 (624650)	624650..625012	+	363	WP_000985972.1	hypothetical protein	-
LTK20_RS03575 (625009)	625009..626175	+	1167	WP_000762527.1	DUF2800 domain-containing protein	-
LTK20_RS03580 (626202)	626202..626759	+	558	WP_000645042.1	DUF2815 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	614851..666226	51375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF2829 (2-69)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9468.72 Da
• Isoelectric Point: 5.7783

>T227745 WP_001025401.1 NZ_CP089159:622922-623167 [Staphylococcus aureus]
MNIQEATKIATKNLVSMTRKDWKESHRTKILPTNDSFLQCIISNSDGTNLIRYWQPSADDLMANDWEVINPTRDQELLKQ
F

Nucleotide

• Length: 246 bp

>T227745 NZ_CP123029:1085056-1085367 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Nucleotide

• Length: 366 bp

>AT227745 NZ_CP089159:c623501-623136 [Staphylococcus aureus]
ATGCCCTTGATATCTGATGAATTTGATACACTTACTAAAGACCAACAATATATCTTGTCCGTACTCTACAAAGATTATTT
AGAATGTGTAAAGTTAGGTTCGGTTAAATTAACCTGCAATAATTTTGGAAGTGCTAAAGATATACATACAAAGTATTTTC
AAAAACTACATTTCGAAGATGTAAAATACGATTTAAATAAACTTAAAAACTCTGGGTTCCTAAACGGCGTGTATGCTAGT
AACACTATTTATCATGTAACAATTTCAGACAAGACTGTTGTTTACTTTGAAAATGAGTTTAAAAACAATTTAAAAAGTAT
CATTGATAGCATTTCTAAAATTGCTTCAATAATTCCTGGTCTCTAG

Structures

Toxin

• AlphaFold DB: A0A4P7P609

Antitoxin