Detailed information of TA system TA227725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1009318..1009835
• Replicon: chromosome
• Accession: NZ_CP089156
• Organism: Staphylococcus aureus strain UNC_SaCF35

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A170NWK0
• Locus tag: LTK19_RS05500
• Protein ID: WP_000251928.1
• Coordinates: 1009318..1009584 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A166LMC5
• Locus tag: LTK19_RS05505
• Protein ID: WP_000584497.1
• Coordinates: 1009584..1009835 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTK19_RS05475 (LTK19_05475)	1004526..1005062	-	537	WP_000024292.1	GNAT family N-acetyltransferase	-
LTK19_RS05480 (LTK19_05480)	1005530..1006354	-	825	WP_000594640.1	formate/nitrite transporter family protein	-
LTK19_RS05485 (LTK19_05485)	1006557..1006739	-	183	WP_000079140.1	hypothetical protein	-
LTK19_RS05490 (LTK19_05490)	1006825..1007292	-	468	WP_000153340.1	SRPBCC domain-containing protein	-
LTK19_RS05495 (LTK19_05495)	1007478..1009025	-	1548	WP_000727771.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
LTK19_RS05500 (LTK19_05500)	1009318..1009584	-	267	WP_000251928.1	Txe/YoeB family addiction module toxin	Toxin
LTK19_RS05505 (LTK19_05505)	1009584..1009835	-	252	WP_000584497.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LTK19_RS05510 (LTK19_05510)	1009999..1010099	-	101	Protein_1049	hypothetical protein	-
LTK19_RS05515 (LTK19_05515)	1010106..1010705	-	600	WP_000162804.1	DsbA family protein	-
LTK19_RS05520 (LTK19_05520)	1010724..1011086	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
LTK19_RS05525 (LTK19_05525)	1011341..1012591	+	1251	WP_001012237.1	FemA/FemB family glycyltransferase FmhA	-
LTK19_RS05530 (LTK19_05530)	1012688..1013419	-	732	WP_000615467.1	amino acid ABC transporter ATP-binding protein	-
LTK19_RS05535 (LTK19_05535)	1013416..1014135	-	720	WP_000479573.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.06 Da
• Isoelectric Point: 10.1628

>T227725 WP_000251928.1 NZ_CP089156:c1009584-1009318 [Staphylococcus aureus]
MVRLNITFSPQAFEDYKYFQQNDKKMVKKINELIKSIDRNGALKGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T227725 NZ_CP123024:1837778-1838032 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9401.54 Da
• Isoelectric Point: 4.3407

>AT227725 WP_000584497.1 NZ_CP089156:c1009835-1009584 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDIVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTVTKD
IDV

Nucleotide

• Length: 252 bp

>AT227725 NZ_CP123024:1837530-1837781 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A170NWK0

Antitoxin

• AlphaFold DB: A0A166LMC5