Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3784343..3785037 | Replicon | chromosome |
| Accession | NZ_CP089142 | ||
| Organism | Escherichia coli strain XD35 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | K0768_RS21770 | Protein ID | WP_001263493.1 |
| Coordinates | 3784343..3784741 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | K0768_RS21775 | Protein ID | WP_000554757.1 |
| Coordinates | 3784744..3785037 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3780003) | 3780003..3780083 | - | 81 | NuclAT_9 | - | - |
| - (3780003) | 3780003..3780083 | - | 81 | NuclAT_9 | - | - |
| - (3780003) | 3780003..3780083 | - | 81 | NuclAT_9 | - | - |
| - (3780003) | 3780003..3780083 | - | 81 | NuclAT_9 | - | - |
| K0768_RS21740 (3779343) | 3779343..3780587 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| K0768_RS21745 (3780679) | 3780679..3781137 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| K0768_RS21750 (3781398) | 3781398..3782855 | + | 1458 | WP_063084593.1 | cytosol nonspecific dipeptidase | - |
| K0768_RS21755 (3782912) | 3782912..3783433 | - | 522 | Protein_3619 | peptide chain release factor H | - |
| K0768_RS21760 (3783432) | 3783432..3783635 | - | 204 | Protein_3620 | RtcB family protein | - |
| K0768_RS21765 (3783881) | 3783881..3784333 | - | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| K0768_RS21770 (3784343) | 3784343..3784741 | - | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| K0768_RS21775 (3784744) | 3784744..3785037 | - | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| K0768_RS21780 (3785089) | 3785089..3786144 | - | 1056 | WP_063084594.1 | DNA polymerase IV | - |
| K0768_RS21785 (3786215) | 3786215..3787138 | - | 924 | WP_001232547.1 | putative lateral flagellar export/assembly protein LafU | - |
| K0768_RS21790 (3787141) | 3787141..3788004 | - | 864 | WP_001169518.1 | flagellar motor stator protein MotA | - |
| K0768_RS21795 (3788017) | 3788017..3788733 | - | 717 | WP_000938723.1 | FliA/WhiG family RNA polymerase sigma factor | - |
| K0768_RS21800 (3788753) | 3788753..3789220 | - | 468 | WP_000725257.1 | flagellar basal body-associated FliL family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T227689 WP_001263493.1 NZ_CP089142:c3784741-3784343 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T227689 NZ_CP123014:93137-93553 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT227689 WP_000554757.1 NZ_CP089142:c3785037-3784744 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT227689 NZ_CP123014:92910-93140 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|