Detailed information of TA system TA227676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1853931..1854433
• Replicon: chromosome
• Accession: NZ_CP089142
• Organism: Escherichia coli strain XD35

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: K0768_RS12235
• Protein ID: WP_000767829.1
• Coordinates: 1854179..1854433 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: K0768_RS12230
• Protein ID: WP_001259255.1
• Coordinates: 1853931..1854182 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0768_RS12205 (1849109)	1849109..1850176	-	1068	WP_000080141.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
K0768_RS12210 (1850176)	1850176..1851246	-	1071	WP_000108941.1	histidinol-phosphate transaminase	-
K0768_RS12215 (1851243)	1851243..1852547	-	1305	WP_001367667.1	histidinol dehydrogenase	-
K0768_RS12220 (1852553)	1852553..1853452	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
K0768_RS12225 (1853598)	1853598..1853648	-	51	WP_001364200.1	his operon leader peptide	-
K0768_RS12230 (1853931)	1853931..1854182	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
K0768_RS12235 (1854179)	1854179..1854433	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
K0768_RS12240 (1854516)	1854516..1855340	+	825	WP_000754737.1	SDR family oxidoreductase	-
K0768_RS12245 (1855386)	1855386..1856315	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
K0768_RS12250 (1856530)	1856530..1856592	+	63	WP_010723108.1	membrane protein YoeI	-
K0768_RS12255 (1856582)	1856582..1857940	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
K0768_RS12260 (1858119)	1858119..1859177	+	1059	WP_000492316.1	thiosulfate utilization transporter TsuA/YeeE	-
K0768_RS12265 (1859191)	1859191..1859418	+	228	WP_000985278.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T227676 WP_000767829.1 NZ_CP089142:1854179-1854433 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T227676 NZ_CP123013:1838730-1838981 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT227676 WP_001259255.1 NZ_CP089142:1853931-1854182 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT227676 NZ_CP123013:1838978-1839232 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0