Detailed information of TA system TA022767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 21481..22057
• Replicon: plasmid pMRAD04
• Accession: NC_010517
• Organism: Methylobacterium radiotolerans JCM 2831

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B1MA03
• Locus tag: MRAD2831_RS63510
• Protein ID: WP_012340153.1
• Coordinates: 21481..21768 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: MRAD2831_RS63515
• Protein ID: WP_041373180.1
• Coordinates: 21773..22057 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRAD2831_RS63490	16540..17757	+	1218	WP_012340148.1	flagellar protein	-
MRAD2831_RS63500	19319..19909	-	591	WP_012340150.1	recombinase family protein	-
MRAD2831_RS63505	20565..21230	-	666	WP_012340152.1	ParA family protein	-
MRAD2831_RS63510	21481..21768	+	288	WP_012340153.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MRAD2831_RS63515	21773..22057	+	285	WP_041373180.1	HigA family addiction module antidote protein	Antitoxin
MRAD2831_RS63520	22435..23250	+	816	WP_012340155.1	hypothetical protein	-
MRAD2831_RS63525	23657..24148	-	492	WP_012340156.1	hypothetical protein	-
MRAD2831_RS63530	24593..25036	+	444	WP_012340157.1	MucR family transcriptional regulator	-
MRAD2831_RS63535	25128..26396	-	1269	WP_012340158.1	IS256 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37743	37743

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-66)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10974.35 Da
• Isoelectric Point: 6.4748

>T22767 WP_012340153.1 NC_010517:21481-21768 [Methylobacterium radiotolerans JCM 2831]
MIQSFADKDTEALHLHDTCHRQWRAFKSVAVRKLDMIDAAVALGDLRSPPGNQLEKLSGDRDGQWSIRINDQWRICFRWG
DIGPENVEIVDYHRG

Nucleotide

• Length: 288 bp

>T22767 NC_010517:21481-21768 [Methylobacterium radiotolerans JCM 2831]
ATGATTCAGAGTTTCGCGGACAAGGATACGGAGGCGCTCCACCTCCACGATACCTGTCACCGGCAGTGGCGTGCCTTTAA
GTCCGTCGCCGTGCGCAAGCTCGATATGATTGATGCCGCAGTCGCGTTGGGCGACCTGCGATCTCCGCCTGGCAATCAGC
TCGAGAAGCTGAGCGGGGATCGCGACGGACAGTGGAGCATCCGCATCAACGACCAGTGGCGCATCTGCTTCAGGTGGGGC
GACATCGGACCGGAGAACGTCGAGATCGTGGACTATCACAGAGGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10658.12 Da
• Isoelectric Point: 6.8809

>AT22767 WP_041373180.1 NC_010517:21773-22057 [Methylobacterium radiotolerans JCM 2831]
MPRLRTHPGEVLREEFMAPLGLSANRLAAEIDVPANRLSEIIRERRGVTADTALRLSIRFGTTPQFWLNLQTAHDLSHAE
AETDLSKVRHYEPV

Nucleotide

• Length: 285 bp

>AT22767 NC_010517:21773-22057 [Methylobacterium radiotolerans JCM 2831]
ATGCCCCGTCTGCGAACGCACCCTGGCGAGGTTCTACGCGAGGAGTTCATGGCGCCGCTCGGCCTCAGCGCCAACCGCCT
CGCAGCTGAGATCGATGTGCCTGCTAACAGGCTAAGCGAGATCATCCGCGAGCGTCGCGGTGTGACCGCAGATACCGCAC
TTCGGCTCAGCATTCGGTTTGGGACGACGCCTCAGTTCTGGCTCAACCTTCAGACCGCCCACGACCTCTCGCATGCAGAG
GCCGAGACGGATCTCTCAAAGGTGCGGCACTACGAGCCGGTGTGA

Structures

Toxin

• AlphaFold DB: B1MA03