Detailed information of TA system TA227668

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 84795..85048
• Replicon: plasmid pXD35004
• Accession: NZ_CP089137
• Organism: Escherichia coli strain XD35

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K0768_RS03080
• Protein ID: WP_001312851.1
• Coordinates: 84899..85048 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	84795..84854 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0768_RS03055 (81522)	81522..82268	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
K0768_RS03060 (82323)	82323..82883	+	561	WP_000139328.1	fertility inhibition protein FinO	-
K0768_RS03065 (83015)	83015..83215	+	201	WP_015059022.1	hypothetical protein	-
K0768_RS03070 (83601)	83601..84200	+	600	WP_032083981.1	PIN domain-containing protein	-
K0768_RS03075 (84262)	84262..84594	+	333	WP_152916585.1	hypothetical protein	-
- (84795)	84795..84854	-	60	NuclAT_1	-	Antitoxin
- (84795)	84795..84854	-	60	NuclAT_1	-	Antitoxin
- (84795)	84795..84854	-	60	NuclAT_1	-	Antitoxin
- (84795)	84795..84854	-	60	NuclAT_1	-	Antitoxin
K0768_RS03080 (84899)	84899..85048	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
K0768_RS03085 (85332)	85332..85580	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-1 / aph(3')-VI / blaTEM-1B / rmtB	-	1..85891	85891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T227668 WP_001312851.1 NZ_CP089137:84899-85048 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T227668 NZ_CP123013:318968-319198 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT227668 NZ_CP089137:c84854-84795 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin