Detailed information of TA system TA227665

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45341..45610
• Replicon: plasmid pXD35004
• Accession: NZ_CP089137
• Organism: Escherichia coli strain XD35

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K0768_RS02850
• Protein ID: WP_001372321.1
• Coordinates: 45485..45610 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	45341..45406 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0768_RS02815	41051..41578	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
K0768_RS02820	41636..41869	+	234	WP_000006003.1	DUF905 family protein	-
K0768_RS02825	41930..43953	+	2024	Protein_54	ParB/RepB/Spo0J family partition protein	-
K0768_RS02830	44022..44456	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
K0768_RS02835	44453..45215	+	763	Protein_56	plasmid SOS inhibition protein A	-
-	45184..45408	+	225	NuclAT_0	-	-
-	45184..45408	+	225	NuclAT_0	-	-
-	45184..45408	+	225	NuclAT_0	-	-
-	45184..45408	+	225	NuclAT_0	-	-
K0768_RS02840	45193..45372	-	180	WP_001309233.1	hypothetical protein	-
-	45341..45406	-	66	-	-	Antitoxin
K0768_RS02845	45394..45543	+	150	Protein_58	plasmid maintenance protein Mok	-
K0768_RS02850	45485..45610	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
K0768_RS02855	45929..46225	-	297	Protein_60	hypothetical protein	-
K0768_RS02860	46525..46821	+	297	WP_001272251.1	hypothetical protein	-
K0768_RS02865	46932..47753	+	822	WP_001234445.1	DUF932 domain-containing protein	-
K0768_RS02870	48050..48697	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
K0768_RS02875	48974..49357	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K0768_RS02880	49548..50234	+	687	WP_015059009.1	PAS domain-containing protein	-
K0768_RS02885	50328..50555	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-1 / aph(3')-VI / blaTEM-1B / rmtB	-	1..85891	85891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T227665 WP_001372321.1 NZ_CP089137:45485-45610 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T227665 NZ_CP123009:c4542927-4542463 [Escherichia coli O155]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT227665 NZ_CP089137:c45406-45341 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin