Detailed information of TA system TA227658

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64858..65112
• Replicon: plasmid pXD35002
• Accession: NZ_CP089135
• Organism: Escherichia coli strain XD35

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K0768_RS01695
• Protein ID: WP_001312851.1
• Coordinates: 64858..65007 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	65051..65112 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0768_RS01660 (60238)	60238..60653	-	416	Protein_71	IS1-like element IS1B family transposase	-
K0768_RS01665 (60902)	60902..61303	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
K0768_RS01670 (61236)	61236..61493	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
K0768_RS01675 (61586)	61586..62239	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
K0768_RS01680 (63177)	63177..64034	-	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
K0768_RS01685 (64027)	64027..64101	-	75	WP_061357229.1	RepA leader peptide Tap	-
K0768_RS01690 (64333)	64333..64590	-	258	WP_001541895.1	replication regulatory protein RepA	-
K0768_RS01695 (64858)	64858..65007	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (65051)	65051..65112	+	62	NuclAT_0	-	Antitoxin
- (65051)	65051..65112	+	62	NuclAT_0	-	Antitoxin
- (65051)	65051..65112	+	62	NuclAT_0	-	Antitoxin
- (65051)	65051..65112	+	62	NuclAT_0	-	Antitoxin
K0768_RS01700 (65454)	65454..65666	-	213	WP_001541896.1	ANR family transcriptional regulator	-
K0768_RS01705 (65801)	65801..66361	-	561	WP_000139310.1	fertility inhibition protein FinO	-
K0768_RS01710 (66416)	66416..67159	-	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / tet(A) / aac(3)-IVa / aph(4)-Ia / floR / mph(A) / erm(B) / sul3	-	1..129210	129210

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T227658 WP_001312851.1 NZ_CP089135:c65007-64858 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T227658 NZ_CP123009:2234228-2234458 [Escherichia coli O155]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT227658 NZ_CP089135:65051-65112 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin