Detailed information of TA system TA227651

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 101260..101686
• Replicon: plasmid pEH13_2
• Accession: NZ_CP089099
• Organism: Klebsiella pneumoniae strain EH13

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LSG42_RS28310
• Protein ID: WP_001372321.1
• Coordinates: 101260..101385 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	101462..101686 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG42_RS28275 (96283)	96283..96510	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
LSG42_RS28280 (96647)	96647..97318	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
LSG42_RS28285 (97512)	97512..97895	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LSG42_RS28290 (98230)	98230..98820	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
LSG42_RS28295 (99117)	99117..99938	-	822	WP_001234445.1	DUF932 domain-containing protein	-
LSG42_RS28300 (100049)	100049..100345	-	297	WP_001272251.1	hypothetical protein	-
LSG42_RS28305 (100645)	100645..100941	+	297	Protein_118	hypothetical protein	-
LSG42_RS28310 (101260)	101260..101385	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LSG42_RS28315 (101327)	101327..101476	-	150	Protein_120	plasmid maintenance protein Mok	-
- (101462)	101462..101686	-	225	NuclAT_0	-	Antitoxin
- (101462)	101462..101686	-	225	NuclAT_0	-	Antitoxin
- (101462)	101462..101686	-	225	NuclAT_0	-	Antitoxin
- (101462)	101462..101686	-	225	NuclAT_0	-	Antitoxin
LSG42_RS28320 (101698)	101698..102417	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
LSG42_RS28325 (102414)	102414..102848	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LSG42_RS28330 (102917)	102917..104940	-	2024	Protein_123	ParB/RepB/Spo0J family partition protein	-
LSG42_RS28335 (105001)	105001..105234	-	234	WP_000006003.1	DUF905 family protein	-
LSG42_RS28340 (105292)	105292..105819	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
LSG42_RS28345 (106121)	106121..106576	+	456	Protein_126	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / erm(42) / sul2 / tet(M) / mph(A) / erm(B) / blaCTX-M-14 / qacE / cmlA1 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..152520	152520

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T227651 WP_001372321.1 NZ_CP089099:c101385-101260 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T227651 NZ_CP123009:1327855-1327962 [Escherichia coli O155]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT227651 NZ_CP089099:c101686-101462 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin