Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 36369..37096 Replicon plasmid pEH13_1
Accession NZ_CP089098
Organism Klebsiella pneumoniae strain EH13

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag LSG42_RS26135 Protein ID WP_000558568.1
Coordinates 36785..37096 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7U3M0L1
Locus tag LSG42_RS26130 Protein ID WP_072199090.1
Coordinates 36369..36788 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSG42_RS26100 (LSG42_26100) 31494..32603 - 1110 WP_000842134.1 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase -
LSG42_RS26105 (LSG42_26105) 32698..33882 - 1185 WP_001039466.1 tetracycline efflux MFS transporter Tet(D) -
LSG42_RS26110 (LSG42_26110) 33978..34586 + 609 WP_023352616.1 tetracycline resistance transcriptional repressor TetR(D) -
LSG42_RS26115 (LSG42_26115) 34633..35337 + 705 WP_001067848.1 IS6-like element IS26 family transposase -
LSG42_RS26120 (LSG42_26120) 35390..35548 + 159 Protein_43 IS110 family transposase -
LSG42_RS26125 (LSG42_26125) 35793..36332 + 540 WP_228295988.1 hypothetical protein -
LSG42_RS26130 (LSG42_26130) 36369..36788 - 420 WP_072199090.1 helix-turn-helix domain-containing protein Antitoxin
LSG42_RS26135 (LSG42_26135) 36785..37096 - 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
LSG42_RS26140 (LSG42_26140) 37307..37687 + 381 Protein_47 gluconate permease -
LSG42_RS26145 (LSG42_26145) 37737..38132 - 396 Protein_48 lactate utilization protein C -
LSG42_RS26150 (LSG42_26150) 38155..39123 + 969 WP_074132802.1 IS5 family transposase -
LSG42_RS26155 (LSG42_26155) 39474..40553 - 1080 WP_040210162.1 RepB family plasmid replication initiator protein -
LSG42_RS26160 (LSG42_26160) 40606..41610 + 1005 WP_000427620.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(D) / aph(6)-Id / aph(3'')-Ib / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrB4 / blaDHA-1 / blaTEM-1B - 1..330084 330084
- inside IScluster/Tn tet(D) - 32698..41610 8912


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T227648 WP_000558568.1 NZ_CP089098:c37096-36785 [Klebsiella pneumoniae]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T227648 NZ_CP123009:c476252-476001 [Escherichia coli O155]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15592.75 Da        Isoelectric Point: 4.4075

>AT227648 WP_072199090.1 NZ_CP089098:c36788-36369 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIEALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT227648 NZ_CP123009:c476004-475750 [Escherichia coli O155]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U3M0L1

References