Detailed information of TA system TA227629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4194036..4194871
• Replicon: chromosome
• Accession: NZ_CP089094
• Organism: Microcystis aeruginosa FACHB-905 = DIANCHI905

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A1X9L1Q2
• Locus tag: LRR78_RS19985
• Protein ID: WP_036399371.1
• Coordinates: 4194036..4194533 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: LRR78_RS19990
• Protein ID: WP_036399368.1
• Coordinates: 4194536..4194871 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRR78_RS19955 (LRR78_19955)	4189139..4190164	+	1026	WP_002732998.1	ABC transporter permease	-
LRR78_RS19960 (LRR78_19960)	4190249..4191319	-	1071	Protein_3960	IS630-like element ISMae27 family transposase	-
LRR78_RS19965 (LRR78_19965)	4191394..4191639	-	246	WP_002740773.1	hypothetical protein	-
LRR78_RS19970 (LRR78_19970)	4191643..4192946	-	1304	Protein_3962	IS5 family transposase	-
LRR78_RS19975 (LRR78_19975)	4192987..4193430	+	444	WP_002740761.1	IS200/IS605 family transposase	-
LRR78_RS19980 (LRR78_19980)	4193467..4193682	-	216	Protein_3964	IS5/IS1182 family transposase	-
LRR78_RS19985 (LRR78_19985)	4194036..4194533	-	498	WP_036399371.1	type II toxin-antitoxin system YhaV family toxin	Toxin
LRR78_RS19990 (LRR78_19990)	4194536..4194871	-	336	WP_036399368.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
LRR78_RS19995 (LRR78_19995)	4195141..4196319	+	1179	WP_002732401.1	AI-2E family transporter	-
LRR78_RS20000 (LRR78_20000)	4197415..4197924	+	510	WP_002732961.1	inorganic diphosphatase	-
LRR78_RS20005 (LRR78_20005)	4198080..4198766	+	687	WP_084990110.1	N-acetylmannosamine-6-phosphate 2-epimerase	-
LRR78_RS20010 (LRR78_20010)	4198804..4198869	-	66	Protein_3970	DUF4164 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (14-151)

Antitoxin

• PrlF antitoxin (10-108)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 19635.32 Da
• Isoelectric Point: 8.3819

>T227629 WP_036399371.1 NZ_CP089094:c4194533-4194036 [Microcystis aeruginosa FACHB-905 = DIANCHI905]
MSIDQPLVINGWSIFAHPLFLEQFEELVTQVEELRHKFPQDYKRKNATKLLAAIAKLAFDTIPEDPTRSEYRQGTALGDD
YKHWFRAKFFQQYRLFFRYHQESKIILLAWVNDQNSKRAFESSTDAYRVFRKMLETGYPPDDWHDLLKEAKSTISRLRKA
AETEI

Nucleotide

• Length: 498 bp

>T227629 NZ_CP123008:c1374025-1373831 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12495.02 Da
• Isoelectric Point: 4.2675

>AT227629 WP_036399368.1 NZ_CP089094:c4194871-4194536 [Microcystis aeruginosa FACHB-905 = DIANCHI905]
MAATMAPWPESTLTDRYQTTIPDSVRKALGLNKRDKICYNIQPDGKVWISRAEGTEENNPVLGKFLNFLAQDMEQNPQHI
QPISSNLVNHVQYLVSGVDLDIDAPLLDEDE

Nucleotide

• Length: 336 bp

>AT227629 NZ_CP123008:1373654-1373832 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTAATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A1X9L1Q2