Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 166488..167023 Replicon plasmid pK21EFM001
Accession NZ_CP089093
Organism Enterococcus faecium strain V13-21-E11-012-001

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag LRL10_RS14105 Protein ID WP_002314366.1
Coordinates 166488..166760 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag LRL10_RS14110 Protein ID WP_002297422.1
Coordinates 166760..167023 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRL10_RS14065 (LRL10_14610) 162045..162688 + 644 Protein_167 transposase -
LRL10_RS14070 (LRL10_14615) 162764..163444 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
LRL10_RS14075 (LRL10_14620) 163478..163618 - 141 WP_231811780.1 hypothetical protein -
LRL10_RS14080 (LRL10_14625) 163693..163854 - 162 WP_002349958.1 hypothetical protein -
LRL10_RS14085 (LRL10_14630) 164114..164662 - 549 WP_002292681.1 hypothetical protein -
LRL10_RS14090 (LRL10_14635) 164663..165520 - 858 WP_002292680.1 ParA family protein -
LRL10_RS14095 (LRL10_14640) 165806..166092 - 287 Protein_173 type II toxin-antitoxin system RelE/ParE family toxin -
LRL10_RS14100 (LRL10_14645) 166082..166411 - 330 WP_002292678.1 AbrB family transcriptional regulator -
LRL10_RS14105 (LRL10_14650) 166488..166760 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
LRL10_RS14110 (LRL10_14655) 166760..167023 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LRL10_RS14115 (LRL10_14660) 167129..167452 - 324 WP_224453712.1 hypothetical protein -
LRL10_RS14120 (LRL10_14665) 167551..168114 - 564 Protein_178 IS6-like element ISS1N family transposase -
LRL10_RS14125 (LRL10_14670) 168194..169356 + 1163 WP_102642289.1 IS3 family transposase -
LRL10_RS14130 (LRL10_14675) 169828..170727 - 900 WP_002303235.1 aldose 1-epimerase family protein -
LRL10_RS14135 (LRL10_14680) 170748..171020 - 273 WP_002305107.1 DUF3884 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..201227 201227
- inside IScluster/Tn - - 153065..169356 16291


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T227621 WP_002314366.1 NZ_CP089093:c166760-166488 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T227621 NZ_CP123006:749306-749860 [Sinorhizobium meliloti]
ATGAGGAAACACATTCCGCCCGCATCGCGACCCGATTCGGAACCGACGTCGCCGGTCGAGCCGATAACGCTCTGGCGCGC
CTTCGTTCCGCGCTGGGCACATATGCCGCTATCCGGCGCGGGTGCCGCTCGCTTCGGTGGCCGCTGGAACCCGATCGGCA
TGCCTGCGATTTATGCTGCGCGCGAGCTTTCCACTGCCTGGGCTGAATATAATCAGGGTTTCGTCCAGCATCCGGCGCTC
ATCGTGCAGCTCGAGTTGCGCGGGGCGAGGCTCGCGGACCTCACGGATGCCAGCGTTCTTCTGGAGCTTGGTGTGGATGA
GGCGATCCATCGCTGCGAATGGCGTGATGCGCTCGACAAAGGCGCGGTGCCGGAGACCCACAGGCTGCAGTCGGAGCTTC
TGGCGCGCGACTATCACGGGGTGATCTATCCATCCTTCATGTCGCCCGGCGGCACCTGCGTCGCGCTCTGGCGCTGGAAC
GGTGCGGAAGAACCGCGTCTCGACGTCATCGATCCCGATGGCCGGCTGCCGAAATCGCCCGCCTCATGGGTTTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT227621 WP_002297422.1 NZ_CP089093:c167023-166760 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT227621 NZ_CP123006:748928-749299 [Sinorhizobium meliloti]
ATGATGGCCGTTGATTTCCAGATAGCTGCAGCCCGTTTCGGGGACGATCATTCCCCGTTTCTCTCGGCCCGCCTTGTCGC
CGACTGGTTGGGCGTCACGCTCGCCGAACTCGCCAAGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAATCCGGCG
CCCGGAAGGTCGACAGCGCCTTGAGCAAGGTCGTCCGCATCCTCGCCATGGCTTCCGAAATGGCGGGCGACGAAGCCCGC
GCCGTCATCTGGTTCAAGCACCAGCCTATCCCCGGCTGGGCCGGCAAGACGGCGTTCGATCTGGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCGGTACGTGCCGGCGTCTACGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References