Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 476215..476810 Replicon chromosome
Accession NZ_CP089068
Organism Pseudomonas aeruginosa strain UNC_PaerCF13

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag LSP21_RS02185 Protein ID WP_003113526.1
Coordinates 476532..476810 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag LSP21_RS02180 Protein ID WP_003111575.1
Coordinates 476215..476520 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSP21_RS02160 (LSP21_02165) 471539..472753 + 1215 WP_109040476.1 restriction endonuclease subunit S -
LSP21_RS02165 (LSP21_02170) 472750..473865 + 1116 WP_109040475.1 AAA family ATPase -
LSP21_RS02170 (LSP21_02175) 473862..475484 + 1623 WP_231800077.1 type I restriction-modification system subunit M -
LSP21_RS02180 (LSP21_02185) 476215..476520 - 306 WP_003111575.1 HigA family addiction module antitoxin Antitoxin
LSP21_RS02185 (LSP21_02190) 476532..476810 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LSP21_RS02190 (LSP21_02195) 476863..476991 - 129 Protein_424 integrase -
LSP21_RS02195 (LSP21_02200) 477139..479367 + 2229 WP_003113525.1 TonB-dependent receptor -
LSP21_RS02200 (LSP21_02205) 479437..480084 - 648 WP_003095021.1 carbonate dehydratase -
LSP21_RS02205 (LSP21_02210) 480146..481384 - 1239 WP_017001387.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T227611 WP_003113526.1 NZ_CP089068:c476810-476532 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T227611 NZ_CP123003:c3102220-3101822 [Sinorhizobium meliloti]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAAAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCCGAAAAATCGGCCTGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACGGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCTCATGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACCAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT227611 WP_003111575.1 NZ_CP089068:c476520-476215 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT227611 NZ_CP123003:c3102453-3102220 [Sinorhizobium meliloti]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAGGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATCAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References