Detailed information of TA system TA022761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1094027..1094642
• Replicon: chromosome
• Accession: NC_010515
• Organism: Burkholderia cenocepacia MC0-3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B1K7F4
• Locus tag: BCENMC03_RS20965
• Protein ID: WP_012338841.1
• Coordinates: 1094027..1094368 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BCENMC03_RS20970
• Protein ID: WP_012338842.1
• Coordinates: 1094355..1094642 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCENMC03_RS20930	1089858..1090277	-	420	WP_011548430.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
BCENMC03_RS20935	1090288..1090614	-	327	WP_006485487.1	thioredoxin family protein	-
BCENMC03_RS20940	1090826..1091530	+	705	WP_012338836.1	flavin reductase family protein	-
BCENMC03_RS20945	1091527..1091706	+	180	WP_012338837.1	hypothetical protein	-
BCENMC03_RS20950	1091729..1092061	+	333	WP_012338838.1	DUF2322 family protein	-
BCENMC03_RS20955	1092168..1093034	+	867	WP_012338839.1	hypothetical protein	-
BCENMC03_RS20960	1093180..1093812	+	633	WP_012338840.1	LysE family translocator	-
BCENMC03_RS20965	1094027..1094368	+	342	WP_012338841.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BCENMC03_RS20970	1094355..1094642	+	288	WP_012338842.1	helix-turn-helix domain-containing protein	Antitoxin
BCENMC03_RS20975	1094724..1095293	-	570	WP_012338843.1	hypothetical protein	-
BCENMC03_RS20980	1095517..1095975	-	459	WP_006486309.1	Lrp/AsnC family transcriptional regulator	-
BCENMC03_RS20985	1096101..1096835	+	735	WP_011548438.1	AzlC family ABC transporter permease	-
BCENMC03_RS20990	1096828..1097136	+	309	WP_011548439.1	AzlD family protein	-
BCENMC03_RS20995	1097328..1098107	+	780	WP_011548440.1	5'/3'-nucleotidase SurE	-
BCENMC03_RS21000	1098195..1099016	-	822	WP_011548441.1	SDR family oxidoreductase	-
BCENMC03_RS21005	1099201..1099620	-	420	WP_011694472.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (20-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-90)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13007.16 Da
• Isoelectric Point: 9.7874

>T22761 WP_012338841.1 NC_010515:1094027-1094368 [Burkholderia cenocepacia MC0-3]
MPTHGYEIQFFNERVAEGVASLPTTLLARYFALADRMERYGPHLGEPHSQSMGNGLYEMRLKGAEGIARVFYCAIVERRI
VMLHCFVKKTPRTPLKELEIARRRLKEVRDGNV

Nucleotide

• Length: 342 bp

>T22761 NC_010515:1094027-1094368 [Burkholderia cenocepacia MC0-3]
ATGCCGACTCATGGTTACGAGATCCAGTTCTTCAATGAGCGTGTAGCGGAAGGCGTAGCGTCGTTGCCGACGACGCTGCT
TGCCCGGTACTTTGCACTCGCGGACCGCATGGAACGGTACGGCCCGCATCTCGGGGAGCCGCACTCCCAATCGATGGGAA
ACGGCCTGTATGAGATGCGACTGAAAGGCGCGGAAGGTATTGCGCGGGTGTTTTACTGCGCGATCGTCGAACGCCGAATC
GTCATGCTCCACTGTTTCGTGAAGAAGACGCCAAGGACGCCGCTGAAAGAACTGGAAATCGCGCGCCGTCGCTTGAAGGA
GGTTCGAGATGGCAACGTATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10394.94 Da
• Isoelectric Point: 9.6538

>AT22761 WP_012338842.1 NC_010515:1094355-1094642 [Burkholderia cenocepacia MC0-3]
MATYKELRERALADPAVRTEYERLNREEFALLDAMLAARREAGLSQADVAARMGTKAPAITRLERALATGQHSPSVDTLR
KYAAACGKTLVISFA

Nucleotide

• Length: 288 bp

>AT22761 NC_010515:1094355-1094642 [Burkholderia cenocepacia MC0-3]
ATGGCAACGTATAAGGAACTACGTGAGCGGGCACTGGCCGATCCCGCGGTGCGCACGGAATACGAACGATTGAATCGCGA
GGAATTCGCGCTGCTCGACGCGATGCTGGCTGCGCGGCGAGAAGCGGGATTGTCGCAAGCCGACGTGGCGGCACGCATGG
GCACGAAAGCGCCCGCCATTACCCGACTCGAGCGCGCGCTCGCAACGGGGCAGCATTCCCCGTCCGTCGATACGCTGCGC
AAGTATGCGGCGGCTTGCGGCAAGACGCTCGTGATCTCGTTCGCCTGA

Structures

Toxin

• AlphaFold DB: B1K7F4