Detailed information of TA system TA227604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2816605..2817286
• Replicon: chromosome
• Accession: NZ_CP089067
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LSP16_RS13365
• Protein ID: WP_015503432.1
• Coordinates: 2816605..2816970 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSP16_RS13370
• Protein ID: WP_003145733.1
• Coordinates: 2816963..2817286 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP16_RS13335 (LSP16_13325)	2812574..2813008	-	435	WP_003116494.1	RidA family protein	-
LSP16_RS13340 (LSP16_13330)	2813229..2814182	+	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
LSP16_RS13345 (LSP16_13335)	2814155..2815039	-	885	WP_003085663.1	LysR substrate-binding domain-containing protein	-
LSP16_RS13350 (LSP16_13340)	2815141..2815566	+	426	WP_003085665.1	VOC family protein	-
LSP16_RS13355 (LSP16_13345)	2815597..2815869	-	273	WP_023104842.1	hypothetical protein	-
LSP16_RS13360 (LSP16_13350)	2816076..2816315	+	240	WP_044060930.1	hypothetical protein	-
LSP16_RS13365 (LSP16_13355)	2816605..2816970	+	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSP16_RS13370 (LSP16_13360)	2816963..2817286	+	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
LSP16_RS13375 (LSP16_13365)	2817662..2818750	-	1089	WP_030046797.1	DUF3396 domain-containing protein	-
LSP16_RS13380 (LSP16_13370)	2818767..2819468	-	702	WP_030046795.1	VRR-NUC domain-containing protein	-
LSP16_RS13385 (LSP16_13375)	2819465..2819983	-	519	WP_010793873.1	PAAR domain-containing protein	-
LSP16_RS13390 (LSP16_13380)	2820157..2820492	-	336	WP_003119438.1	TM2 domain-containing protein	-
LSP16_RS13395 (LSP16_13385)	2820735..2821373	-	639	WP_003119439.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2796803..2863216	66413

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T227604 WP_015503432.1 NZ_CP089067:2816605-2816970 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T227604 NZ_CP123003:653947-654210 [Sinorhizobium meliloti]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT227604 WP_003145733.1 NZ_CP089067:2816963-2817286 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT227604 NZ_CP123003:654207-654524 [Sinorhizobium meliloti]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCGAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG