Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 2043385..2043980 Replicon chromosome
Accession NZ_CP089067
Organism Pseudomonas aeruginosa strain UNC_PaerCF19

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag LSP16_RS09655 Protein ID WP_219832885.1
Coordinates 2043385..2043663 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LSP16_RS09660 Protein ID WP_003113527.1
Coordinates 2043675..2043980 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSP16_RS09635 (LSP16_09625) 2038811..2040049 + 1239 WP_003113524.1 dipeptidase -
LSP16_RS09640 (LSP16_09630) 2040111..2040758 + 648 WP_003095021.1 carbonate dehydratase -
LSP16_RS09645 (LSP16_09635) 2040828..2043056 - 2229 WP_004352671.1 TonB-dependent receptor -
LSP16_RS09650 (LSP16_09640) 2043204..2043332 + 129 Protein_1902 integrase -
LSP16_RS09655 (LSP16_09645) 2043385..2043663 + 279 WP_219832885.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LSP16_RS09660 (LSP16_09650) 2043675..2043980 + 306 WP_003113527.1 HigA family addiction module antitoxin Antitoxin
LSP16_RS09665 (LSP16_09655) 2044658..2044876 + 219 WP_134593965.1 hypothetical protein -
LSP16_RS09670 (LSP16_09660) 2044952..2046022 + 1071 WP_231786496.1 reverse transcriptase family protein -
LSP16_RS09675 (LSP16_09665) 2046027..2046365 - 339 WP_134593962.1 hypothetical protein -
LSP16_RS09680 (LSP16_09670) 2046812..2047084 + 273 WP_003115921.1 hypothetical protein -
LSP16_RS09685 (LSP16_09675) 2047077..2047292 + 216 WP_231786497.1 hypothetical protein -
LSP16_RS09690 (LSP16_09680) 2047296..2047583 + 288 WP_231786498.1 DUF5447 family protein -
LSP16_RS09695 (LSP16_09685) 2047591..2048235 + 645 WP_003133746.1 DNA cytosine methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10671.25 Da        Isoelectric Point: 9.3867

>T227602 WP_219832885.1 NZ_CP089067:2043385-2043663 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRRGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T227602 NZ_CP123002:183831-184052 [Neorhizobium petrolearium]
GTGTTTGCTGACCCCGTTCTTGCCGGGATTGTTTGGCGGGACATTGAGGCGCTATTCGTCGCATGTGGGGCAGAAATCAC
GGAAGGGCAAGGCTCGCGGGTGCGGGTCGTCCTGAACGGCGTGCGCGCGGTTTTCCATCGCCCGCATCCCCAGAAGGAAA
CCGACAAGGGAGGGGTAAAATCCGTCCGGCGCTTCCTGCTCGAAGCGGAGATACAACCATGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT227602 WP_003113527.1 NZ_CP089067:2043675-2043980 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp

>AT227602 NZ_CP123002:184049-184390 [Neorhizobium petrolearium]
ATGACCACAATGCACTACAAGGGCTACGAAGCTGTTGTTGAGTTCGATGAGGACGCCGATATTTTCCACGGTGAGGTCGT
CAACATGCGCGATGTCATTACCTTTCAGGGAACTTCGGCGGCCGAGCTGAAGCAGGCAATGGCGGATTCCATTGAGGATT
ACCTGGTGTTCTGCAAAGAGCGTGGCGAGGAACCCGAAAAGCCTTTTTCCGGCCAATTCGTTGTCAGGACCGAACCTAGT
ATCCACAAAGCTGTGTCGAGTGCCGCACGACGAGAGGGCGTAAGTCTCAACAAATGGGTGACGCAGGCGTTGGAACGCGC
CGTTTCGCGGAAGGTCGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References