Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 6117007..6117629 Replicon chromosome
Accession NZ_CP089065
Organism Pseudomonas aeruginosa strain UNC_PaerCF34

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag LSP20_RS28500 Protein ID WP_023910260.1
Coordinates 6117315..6117629 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LSP20_RS28495 Protein ID WP_023910258.1
Coordinates 6117007..6117312 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSP20_RS28450 (LSP20_28460) 6112876..6113262 + 387 WP_231822251.1 LuxR C-terminal-related transcriptional regulator -
LSP20_RS28455 (LSP20_28465) 6113265..6113498 + 234 WP_231822252.1 hypothetical protein -
LSP20_RS28460 (LSP20_28470) 6113596..6113826 + 231 WP_023094535.1 Arc family DNA-binding protein -
LSP20_RS28465 (LSP20_28475) 6113829..6114164 + 336 WP_003129612.1 hypothetical protein -
LSP20_RS28470 (LSP20_28480) 6114161..6115129 + 969 WP_231822253.1 hypothetical protein -
LSP20_RS31265 6115126..6115248 + 123 WP_277990625.1 hypothetical protein -
LSP20_RS28475 (LSP20_28485) 6115248..6115439 + 192 WP_003129607.1 hypothetical protein -
LSP20_RS28480 (LSP20_28490) 6115612..6116301 + 690 WP_231822254.1 hypothetical protein -
LSP20_RS28485 (LSP20_28495) 6116294..6116647 + 354 WP_231822255.1 hypothetical protein -
LSP20_RS28490 (LSP20_28500) 6116644..6116946 + 303 WP_031685410.1 hypothetical protein -
LSP20_RS28495 (LSP20_28505) 6117007..6117312 - 306 WP_023910258.1 helix-turn-helix domain-containing protein Antitoxin
LSP20_RS28500 (LSP20_28510) 6117315..6117629 - 315 WP_023910260.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LSP20_RS28505 (LSP20_28515) 6117626..6117973 - 348 WP_231822256.1 hypothetical protein -
LSP20_RS28510 (LSP20_28520) 6118208..6118741 - 534 WP_231822257.1 PIN domain-containing protein -
LSP20_RS28515 (LSP20_28525) 6118731..6119186 - 456 WP_231822258.1 hypothetical protein -
LSP20_RS28520 (LSP20_28530) 6119226..6120388 + 1163 WP_231822259.1 IS3-like element IS222 family transposase -
LSP20_RS28525 (LSP20_28535) 6120363..6120806 - 444 WP_231822260.1 hypothetical protein -
LSP20_RS28530 (LSP20_28540) 6120962..6121612 - 651 WP_023910263.1 BRCT domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 6075380..6123071 47691


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-101)

Antitoxin

Cro/C1-type helix-turn-helix domain

(36-72)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11776.54 Da        Isoelectric Point: 9.4385

>T227600 WP_023910260.1 NZ_CP089065:c6117629-6117315 [Pseudomonas aeruginosa]
MIFIETPVFTKRILALVDDETYRKLQEDLTLHPDAGVVIEGTGGVRKIRIAANGHGKRGGARVIYYHFTSASQIAFLLAY
DKASQEDLTADQKKMLRQIVENWR

Download         Length: 315 bp

>T227600 NZ_CP123001:416585-416875 [Neorhizobium petrolearium]
ATGACCGGCTTCATACTGTCCCCCGCCGCGCAGGCCGATGTCGAGCGGATTTGGGACTACACGGCGACACGGTGGAATGT
CGATCAGGCAGAGCGATATATCCAGAATATTAGGGACGCATGCCGCGCACTGGCCGCCGGCACCCGCGTCAGCCGCCCCG
TCGATATTCGCGAAGGATACCGGAAAGTCTCGGTCGGCTCCCACTTCATCTATTTCAAAAGCAACGATACGGGGCAGATC
GTCGTTATCCGCATCCTTCATCAAAGAATGGACGTCGATAACCGTCTTTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11316.84 Da        Isoelectric Point: 7.2077

>AT227600 WP_023910258.1 NZ_CP089065:c6117312-6117007 [Pseudomonas aeruginosa]
MDKELFAELVESVTQMDEIVRGERQPSREFHVDAVKVKEIRRATGLSQAKFARKIDVAVGTLRNWEQGRRDPEGPARALL
RAIDNDPVHVLAALNGGPRES

Download         Length: 306 bp

>AT227600 NZ_CP123001:416340-416588 [Neorhizobium petrolearium]
ATGGCTCGAAACACATCCATTTCGCTTGGCGATCATTTCGCCACTTTCATTGACACGCAAGTCCAGGCGGGGCGCTACGG
TTCGGCCAGCGACGTGGTGCGTGCAGGCCTGCGCCTGCTCGAAGAGCACGAGGCCAAAGTGAAGGCATTGCATGCCGCGT
TGATCGAAGGTGAAGAATCCGGCCCTGCAACCGCCTTCGATTTCGACGCCTTCAATGCGCGCAAGCGCGCCGCATTTGAG
GCGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References