Detailed information of TA system TA227595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1754415..1755010
• Replicon: chromosome
• Accession: NZ_CP089065
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF34

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: LSP20_RS08120
• Protein ID: WP_003113526.1
• Coordinates: 1754415..1754693 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSP20_RS08125
• Protein ID: WP_003099268.1
• Coordinates: 1754705..1755010 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP20_RS08100 (LSP20_08100)	1749841..1751079	+	1239	WP_009316205.1	C69 family dipeptidase	-
LSP20_RS08105 (LSP20_08105)	1751141..1751788	+	648	WP_003095021.1	carbonate dehydratase	-
LSP20_RS08110 (LSP20_08110)	1751858..1754086	-	2229	WP_003099265.1	TonB-dependent receptor	-
LSP20_RS08115 (LSP20_08115)	1754234..1754362	+	129	Protein_1598	integrase	-
LSP20_RS08120 (LSP20_08120)	1754415..1754693	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSP20_RS08125 (LSP20_08125)	1754705..1755010	+	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
LSP20_RS08130 (LSP20_08130)	1755347..1755760	+	414	WP_052188955.1	hypothetical protein	-
LSP20_RS08135 (LSP20_08135)	1755966..1756496	+	531	WP_071535170.1	ATP-binding protein	-
LSP20_RS08140 (LSP20_08140)	1756500..1757552	+	1053	WP_051428817.1	hypothetical protein	-
LSP20_RS08145 (LSP20_08145)	1757530..1757928	+	399	WP_051428818.1	ASCH domain-containing protein	-
LSP20_RS08150 (LSP20_08150)	1757951..1759501	+	1551	WP_071535171.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T227595 WP_003113526.1 NZ_CP089065:1754415-1754693 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T227595 NZ_CP123000:c2827219-2826722 [Neorhizobium petrolearium]
GTGACCCTCAGTGCACCGGCCCCCCTGGCCGATCATCATGAACTGGCCGATTTCAATTCCGGTGTCCCTGAACTGGACGA
CTGGCTGCGTCGCCGCGCCCGCGCCAATCAAGCAGGCGGCGCATCGCGTACTTTCGTCGTGTGCGAGGGAATCGGCGTTA
TCGCCTATTACGCGCTCGCCTCGGGCGCGGTAAAACAGCCGGAAGCACCTGGCCGTTTTCGGCGCAATATGCCGGATCCA
ATCCCAGTCGCTGTGCTTGGTCGCCTCGCCATTGATCAATCCTATCAGGGGCGTGGTATCGGCAGGGCATTAGTGCGCGA
CGCGGGCCTGCGCTTGCGTAATGCCGCTGAAGTACTCGGCATCCGCGGCGTGCTGGTCCATGCGATTTCCGATGACGCAC
GAGCCTTCTACGAGGCCGTCGGCTTCCTGCCTTCTCCGTCCGACCCCATGATGCTGATGGTCGGATTGCACGACCTCAAC
GACGCGCTGAACGGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT227595 WP_003099268.1 NZ_CP089065:1754705-1755010 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT227595 NZ_CP123000:c2827512-2827216 [Neorhizobium petrolearium]
ATGCCGACCTCACGCCCGAGTAAAAGTCCAGACGCAAGTGTGCCGCTGAATATGCGCATCAAGCCTGCGACCCGCAACTT
GATCGATCGCGCCGCCGAGTTGCTCGGTAAAACGCGCACGGATTTCATGTTGGAAGCTTCCGAGCGCCGTGCCGAGGAAG
TGCTGCTCGACCGTATTATCTTCACGGTAAGCCCCGAAATCTTTGCCGAATACCTTGCCCGGCTTGATGCGCCCGCCCCG
CTCAATGAGCGTCTAAAACGCACCATGTCGACCAAAGCGCCGTGGGATGAGGCGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3