Detailed information of TA system TA227591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1779762..1780357
• Replicon: chromosome
• Accession: NZ_CP089064
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF35

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: LSP22_RS08380
• Protein ID: WP_003113526.1
• Coordinates: 1780079..1780357 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSP22_RS08375
• Protein ID: WP_003133769.1
• Coordinates: 1779762..1780067 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP22_RS08350 (LSP22_08350)	1775587..1775877	-	291	WP_023110435.1	DUF5447 family protein	-
LSP22_RS08355 (LSP22_08355)	1776089..1776361	-	273	WP_003115921.1	hypothetical protein	-
LSP22_RS08360 (LSP22_08360)	1776808..1777146	+	339	WP_031629305.1	hypothetical protein	-
LSP22_RS08365 (LSP22_08365)	1777151..1778221	-	1071	WP_023110436.1	reverse transcriptase family protein	-
LSP22_RS08370 (LSP22_08370)	1778297..1778515	-	219	WP_031629306.1	hypothetical protein	-
LSP22_RS08375 (LSP22_08375)	1779762..1780067	-	306	WP_003133769.1	HigA family addiction module antitoxin	Antitoxin
LSP22_RS08380 (LSP22_08380)	1780079..1780357	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSP22_RS08385 (LSP22_08385)	1780410..1780538	-	129	Protein_1651	integrase	-
LSP22_RS08390 (LSP22_08390)	1780686..1782914	+	2229	WP_021264080.1	TonB-dependent receptor	-
LSP22_RS08395 (LSP22_08395)	1782984..1783631	-	648	WP_003095021.1	carbonate dehydratase	-
LSP22_RS08400 (LSP22_08400)	1783693..1784931	-	1239	WP_231822122.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T227591 WP_003113526.1 NZ_CP089064:c1780357-1780079 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T227591 NZ_CP123000:1637021-1637302 [Neorhizobium petrolearium]
ATGAGCTATGAGCTCGCCTTTCTCGACGTTGCTTTGAAGGAGTGGCGAAAGCTCGACGCCAATATCCGCGATCAATTCAG
GAAGAAGCTTGCCGAGCGGCTTGAAAATCCGCGCGTGCCCTCCGCCCAGCTTTACGGCGCCAAGGACCGCTACAAGGTCA
AGCTCAGAAGCGCCGGTTATCGTCTCGTCTACGAGGTCCGCGATGCGCAACTGATCGTGCTAGTGATCGCAGTTGGCCGG
CGTGACCGGAATGCCGTCTACAAGGCGGCCGAGAAGCGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT227591 WP_003133769.1 NZ_CP089064:c1780067-1779762 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT227591 NZ_CP123000:1636783-1637031 [Neorhizobium petrolearium]
ATGGTTAGCGTTGTCCTTGCCGAAGTGACAGCCAGCGTATCGGAACTGAAGCGCAATCCAATGGGAACCGTCGCCGCAGG
CGAGGGAGCCCCGGTCGCCATCCTGAACCGCAACGAGCCGGCCTTTTATTGCATTCCCGCCAAGGCTTACGAAGTCATGA
TGGATAGGCTCGAAGACCTTGAACTCAATGCGCTGGCGGACAGCCGGGCAGGGGAAGCCGTCGTCAAGGTGACGCTGGAT
GAGCTATGA

Structures

Toxin

• AlphaFold DB: V6ALY3